PGI-RO

Phosphoglucose Isomerase (PGI)

from yeast

• Synonyme(s) : glucose-6-phosphate isomerase
• Numéro de classification (Commission des enzymes): 5.3.1.9 (BRENDA, IUBMB)
• Code UNSPSC : 12352204

Propriétés

• Source biologique: yeast
• Niveau de qualité: 100
• Forme: suspension
• Activité spécifique: ~350 units/mg protein (at 25 °C with fructose-6-P as the substrate.)
• Conditionnement: pkg of 1 mL (10127396001 [2 mg]); pkg of 1 mL (10128139001 [10 mg])
• Fabricant/nom de marque: Roche
• pH optimal: 7.0-7.6
• Température de stockage: 2-8°C

Description

Description générale

Phosphoglucose Isomerase is an enzyme which possesses diagnostic potential when fractionated by cellulose acetate electrophoresis. This cytosolic enzyme plays an important role during glycolysis and glucogenesis. It acts as an tumor-secreted cytokine and autocrine motility factor. It is used as neurotrophic factor, maturation factor and as serine proteinase inhibitor.

Spécificité

Phosphoglucose isomerase (PGI) is specific for G6P (K_m = 0.7 mM) and fructose-6-phosphate (F6P).

Application

Isomerization of ketoses to aldoses.

Qualité

Contaminants: <0.01% F6P-K, GR, 6-PGDH and PGluM, each, <0.2% β-fructosidase

Forme physique

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6

Notes préparatoires

Storage conditions (working solution): A solution of PGI in 20 mM Tris, pH 8.1, containing 0.2 mg BSA/ml, is stable approx. 1 week at 2 to 8 °C.

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): No data available
• Point d'éclair (°C): No data available