LMDH-RO

L-Malate Dehydrogenase (L-MDH)

from pig heart (mitochondrial)

• Numéro de classification (Commission des enzymes): 1.1.1.37 (BRENDA, IUBMB)
• Code UNSPSC : 12352202

Propriétés

• Source biologique: Porcine heart (mitochondrial)
• Niveau de qualité: 100
• Forme: solution; suspension
• Activité spécifique: ~1200 units/mg protein (At 25 °C with oxaloacetate as the substrate.)
• Conditionnement: pkg of 1 mL (10127248001 solution); pkg of 1 mL (10127256001 suspension); pkg of 5 mL (10127914001suspension)
• Fabricant/nom de marque: Roche
• Technique(s): activity assay: suitable
• Couleur: white
• pH optimal: 7.4-7.5(reduction of oxaloacetate); 9.2-9.5(oxidation of malate)
• Solubilité: water: miscible
• Adéquation: suitable for Western blot
• Numéro d'accès NCBI: NM_001244153.1
• Numéro d'accès UniProt: P00346
• Application(s): life science and biopharma
• Activité étrangère: Fumarase <0.01%; GIDH 0.001%; GOT 0.001%; GPT 0.00185%; LDH <0.01%; NADH oxidase <0.001%
• Conditions d'expédition: wet ice
• Température de stockage: 2-8°C
• Informations sur le gène: Porcine ... MDH2(397039)

Description

Description générale

L-malate:NAD⁺ oxidoreductase
The L-malate dehydrogenase enzyme is a nuclear gene product that is synthesized with a 24-residue amino-terminal signal peptide. This peptide is proteolytically cleaved during the translocation of the enzyme to the mitochondrial matrix.

Application

The enzyme L-malate dehydrogenase from pig heart has been used to measure PEPCK (phosphoenolpyruvate carboxykinase) activity. The oxaloacetate, produced by PEPCK, is reduced to malate via the oxidation of NADH, which in turn is measured at 340 nm using a spectrophotometer. The enzyme has also been used to measure oxaloacetate by measuring the reduction in NADH spectroscopically at 340 nm.

Actions biochimiques/physiologiques

The enzyme L-malate dehydrogenase from pig heart catalyzes the oxidation of L-malate to oxaloacetate. The enzyme is an NAD-dependent mitochondrial dehydrogenase that functions in the tricarboxylic acid cycle. It is a component of the malate-aspartate shuttle that transports reducing equivalents across the inner mitochondrial membrane in the form of malate.

Qualité

Contaminants: <0.002% GOT, <0.01% fumarase and LDH, each luM each, <0.02% HK, <0.002% PGI

Forme physique

Solution in 50% glycerol (v/v), pH approximately 7

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6

Notes préparatoires

Activator: – phosphate
– arsenate
– Mg²⁺
– Zn²⁺

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): No data available
• Point d'éclair (°C): No data available