10165875001

Glucose-6-Phosphate Dehydrogenase (G6P-DH)

from Leuconostoc mesenteroides

• Synonyme(s) : G6P-DH, Glucose-6-Phosephate Dehydrogenase
• Numéro de classification (Commission des enzymes): 1.1.1.49 (BRENDA, IUBMB)
• Code UNSPSC : 12352204

Propriétés

• Source biologique: bacterial (Leuconostoc mesenteroides)
• Niveau de qualité: 100
• Forme: solution; suspension
• Activité spécifique: ~550 units/mg protein (At 25 °C (650 U/mg at 30 °C) with glucose-6-P and NAD as the substrates.)
• Poids mol.: dimer 110 kDa
• Conditionnement: pkg of 1 mL (1,000 U)
• Fabricant/nom de marque: Roche
• Concentration: ≥0.1-1.0 % (w/w)
• Technique(s): activity assay: suitable
• Couleur: white
• pH optimal: 7.0-8.5(maximal activity at 7.8)
• Solubilité: water: miscible
• Numéro d'accès NCBI: AAA25265
• Numéro d'accès UniProt: P11411
• Application(s): life science and biopharma
• Activité étrangère: 6-PGDH <0.001%; CK <0.001%; GR <0.01%; HK <0.05%; NADH oxidase <0.02%; PGI <0.01%
• Conditions d'expédition: wet ice
• Température de stockage: 2-8°C

Description

Description générale

Glucose-6-Phosphate Dehydrogenase (G6PD) is a ubiquitous enzyme which acts as a catalyst in producing pentose. It also produces NADPH for various biosynthetic and detoxification reactions. Sometimes G6PD provides an alternative to main glycolytic pathway for glucose utilization. It also has its application as DNA markers on X-chromosome.1

Spécificité

Specificity: At pH 7.8, 25 °C: G6P-DH from Leuconostoc (LG6PDH) is highly specific for D-glucose-6-phosphate (K_m = 36 μM, NADP as coenzyme; 64 μM, NAD as coenzyme), but will use either NADP (K_m = 7.4 μM; relative rate 1.0) or NAD (K_m = 115 μM; relative rate 1.8) as coenzyme.
LG6P-DH does not react with fructose-6-phosphate, fructose-1,6-biphosphate, glucose-1-phosphate or ribose-1-phosphate. LG6P-DG will oxidize 2-deoxy-glucose-6-phosphate with NADP, but not with NAD as coenzyme. There is a slow reaction with D-glucose.
Heat inactivation: The ammonium sulfate suspension is not inactivated when heated to temperatures ≤ 50 °C for 10 minutes. At temperatures > 60 °C the enzyme is rapidly inactivated.

Application

Component of cofactor recycling systems for NADPH.

It was used in enzymatic determination of C6 phosphorylation of glycogen.

Qualité

Contaminants: <0.001% CK, <0.01% GR and PGI each, <0.02% “NADH oxidase”, <0.05% HK, <0.001% 6-PGDH

Définition de l'unité

Unit Conversion: One unit (U) [+25 °C] ≈ 1.18 U [+30 °C] ≈ 1.45 U [+37 °C], all with NAD as coenzyme

Unit Definition: One unit (U) LG6P-DH oxidizes 1 mol of glucose-6-phosphate and reduces 1 mol of NAD in 1 minute at +25 °C and pH 7.8.

Forme physique

Solution of 1,000 U in 1 ml 3.2 M ammonium sulfate, pH approximately 6

Notes préparatoires

Activator: HCO^3- (≤ 0.3 M) activates slightly.

Remarque sur l'analyse

Absorbance of purified enzyme: 1.15 (1 mg enzyme/ml, 280.5 nm)

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): does not flash
• Point d'éclair (°C): does not flash