11418467001

Chymotrypsin Sequencing Grade

from bovine pancreas

• Synonyme(s) : chymotrypsin, protease
• Numéro de classification (Commission des enzymes): 3.4.21.1 (BRENDA, IUBMB)
• Code UNSPSC : 12352204

Propriétés

• Source biologique: bovine pancreas
• Niveau de qualité: 100
• Pureté: ≥90%
• Forme: lyophilized (salt-free)
• Activité spécifique: ≥70 units/mg protein (at 25 °C, with ATEE as a substrate)
• Poids mol.: M_r 25 kDa
• Conditionnement: pkg of 4 × 25 μg
• Fabricant/nom de marque: Roche
• Technique(s): protein sequencing: suitable
• pH optimal: 7.0-9.0
• Conditions d'expédition: wet ice
• Température de stockage: 2-8°C

Description

Description générale

Chymotrypsin Sequencing Grade is isolated as a specific protease in pure form from bovine pancreas.

Spécificité

Serine endopeptidase that specifically hydrolyzes peptide bonds at the C-terminial of Tyr, Phe, and Trp. Leu, Met, Ala, Asp, and Glu are cleaved at a lower rate. Acts also upon amides and esters of susceptible amino acids. The specificity of Chymotrypsin Sequencing Grade is tested with melittin as a substrate.

Application

Use Chymotrypsin Sequencing Grade for the hydrolysis of proteins by chymotrypsin alone or in combination with other proteases. It is suitable for peptide mapping, fingerprinting, and sequence analysis.

Qualité

Purity: Free of impurities that may interfere with the specific cleavage or separation of peptides in reversed-phase HPLC.

Notes préparatoires

Working concentration: The recommended amount of enzyme is 1/200 to 1/20 of the quantity of protein by weight.
Storage conditions (working solution): 2 to 8 °C
A solution in 1 mM HCl can be stored for up to one week at 2 to 8 °C.
Inhibitors: Aprotinin, DFP, PMSF, phenothiazine-N-carbonyl chloride, TPCK, ZPCK, α2-macroglobulin, α1-antitrypsin, soybean trypsin inhibitor, and chymostatin. No inhibition by APMSF.

Reconstitution

Dissolve lyophilized chymotrypsin sequencing grade in 1mM HCl. The proteins to be sequenced are dissolved in digestion buffer (100mM Tris-HCl, 10mM CaCl₂, pH 7.8).

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Informations sur la sécurité

• Pictogrammes: GHS07,GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H315,H319,H334,H335
• Conseils de prudence: P261 - P264 - P280 - P284 - P304 + P340 + P312 - P342 + P311
• Classification des risques: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Organes cibles: Respiratory system
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable