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MAB1393Z

Sigma-Aldrich

Anti-PECAM-1 Antibody, clone TLD-3A12, azide free

clone TLD-3A12, Chemicon®, from mouse

Synonyme(s) :

CD31, MAB1393

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

100

Forme d'anticorps

affinity purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

TLD-3A12, monoclonal

Espèces réactives

rat

Fabricant/nom de marque

Chemicon®

Technique(s)

ELISA: suitable
flow cytometry: suitable
immunohistochemistry: suitable
western blot: suitable

Isotype

IgG1

Adéquation

not suitable for immunohistochemistry (Paraffin)

Numéro d'accès UniProt

Q3SWT0

Conditions d'expédition

dry ice

Modification post-traductionnelle de la cible

unmodified

Spécificité

Rat PECAM-1. Antibody gives positive reaction at high dilutions against recombinant rat PECAM-1, confirming specificity.

Immunogène

Activated, Lewis rat derived microglial cells.

Application

Anti-PECAM-1 Antibody, clone TLD-3A12, azide free detects level of PECAM-1 & has been published & validated for use in ELISA, FC, WB, IH.
Flow Cytometry: 1:50-1:100

Immunohistology: frozen sections (-20°C methanol fixation), not recommended for traditional formalin fixed tissues.

ELISA

Western Blotting: 1:500. MW = 130-140 kDa

Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
Adhesion (CAMs)

Forme physique

Format: Purified
Purified immunoglobulin by affinity chromatography on Protein G. Liquid in phosphate buffered saline containing no preservatives.

Stockage et stabilité

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Remarque sur l'analyse

Control
POSITIVE CONTROL: Activated microglial cells.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Characterization of microglia and macrophages in the central nervous system of rats: definition of the differential expression of molecules using standard and novel monoclonal antibodies in normal CNS and in four models of parenchymal reaction.
Flaris, N A, et al.
Glia, 7, 34-40 (1993)
An interferon-inducible molecule on brain endothelium which controls lymphocyte adhesion mediated by integrins.
Male, D, et al.
Immunology, 84, 453-460 (1995)
D L Livant et al.
Cancer research, 60(2), 309-320 (2000-02-10)
Using naturally serum-free SU-ECM basement membranes as invasion substrates showed that plasma fibronectin was necessary to stimulate invasion by DU 145 human and metastatic MATLyLu (MLL) rat prostate carcinoma cells. This activity mapped to the PHSRN sequence, which induced invasion
Yingchao Shi et al.
PLoS biology, 18(5), e3000705-e3000705 (2020-05-14)
Modeling the processes of neuronal progenitor proliferation and differentiation to produce mature cortical neuron subtypes is essential for the study of human brain development and the search for potential cell therapies. We demonstrated a novel paradigm for the generation of

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