Accéder au contenu
Merck
Toutes les photos(1)

Key Documents

F1903

Sigma-Aldrich

Fibronectin 40 kDa α Chymotryptic Fragment (Heparin-binding region), Human purified

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme


About This Item

Code UNSPSC :
12352202
eCl@ss :
32160405
Nomenclature NACRES :
NA.75

Source biologique

human

Niveau de qualité

Forme

lyophilized

Poids mol.

40 kDa

Fabricant/nom de marque

Chemicon®

Concentration

1 mg/mL

Technique(s)

cell culture | mammalian: suitable

Entrée

sample type epithelial cells
sample type: human embryonic stem cell(s)
sample type mesenchymal stem cell(s)
sample type hematopoietic stem cell(s)
sample type pancreatic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type neural stem cell(s)

Numéro d'accès UniProt

Conditions d'expédition

dry ice

Température de stockage

−20°C

Informations sur le gène

human ... FN1(2335)

Description générale

Heparin-binding 40 kDa fragment of human plasma fibronectin purified from chymotryptic digest of human plasma fibronectin. This preparation contains the CS-1 heparin-binding region of fibronectin. Heparin-binding fragment promotes cytoskeleton organization in integrin signaling. This preparation shows one major band at 40 kDa in SDS-PAGE with no higher molecular weight species present. Smaller proteolytic fragments may be present.

Human sourced raw material used in the production of this product tested negative for hepatitis B virus, hepatitis C virus (HCV), human immunodeficiency virus type-1 (HIV-1) and type-2 (HIV-2), human T-cell lymphotropic virus (HTLV-1 and HTLV-2) and Treponema pallidum. Handle as if potentially infectious.
Product Source: Human

Application

ELISA

Affinity chromatography

Possibly for molecular mapping of fibronectin function

Cell attachment and integrin signaling assays

Optimal working dilutions must be determined by the end user.

Forme physique

Purified by gelatin-Sepharose and heparin-Sepharose column chromatography. Lyophilized from sodium citrate buffered saline, 20mM, NaCl 150mM, pH 7.4. Reconstitute with distilled water.

Stockage et stabilité

Maintain lyophilized material at -20°C for up to one year from date of receipt. After reconstitution, maintain at -20°C for up to six months in undiluted aliquots. Avoid repeated freeze/thaw cycles.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Alignment of biologically active domains in the fibronectin molecule
Ruoslahti, E. et al.
The Journal of Biological Chemistry, 256, 7277-7281 (1981)
Location of the cell-attachment site in fibronectin with monoclonal antibodies and proteolytic fragments of the molecule.
Pierschbacher, M D, et al.
Cell, 26, 259-267 (1981)

Articles

Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

Protocoles

Dilute fibronectin to the desired concentration. Optimum conditions for attachment are dependent on cell type and application. The typical coating concentration is 1 – 5 ug/cm2.Fibronectin coating protocol, products, and FAQs at sigmaaldrich.com

Dilute fibronectin to the desired concentration. Optimum conditions for attachment are dependent on cell type and application. The typical coating concentration is 1 – 5 ug/cm2.Fibronectin coating protocol, products, and FAQs.

This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique