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F0287

Sigma-Aldrich

Fibronectine

lyophilized powder, 70 kDa

Synonyme(s) :

Fibronectin

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About This Item

Numéro MDL:
MFCD00131062
Code UNSPSC :
12352202
eCl@ss :
42020141
Nomenclature NACRES :
NA.75

Source biologique

human plasma

Niveau de qualité

200

Pureté

>90% (SDS-PAGE)

Forme

lyophilized powder

Poids mol.

70 kDa

Conditionnement

pkg of 0.5 mg

Technique(s)

cell culture | mammalian: suitable

Impuretés

HIV and HBsAg, source material tested negative
 Small proteolytic fragments, may contain traces

Solubilité

water: soluble ≥0.500 mg/mL, clear to slightly hazy, colorless

Numéro d'accès UniProt

P02751

Conditions d'expédition

ambient

Température de stockage

−20°C

Informations sur le gène

human ... FN1(2335)

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Description générale

Fibronectin 1 is a glycoprotein of the extracellular matrix that is coded by FN1 gene. It is expressed in the plasma and at the cell surface. FN1 is mapped to human chromosome 2q35. Proteolytic fragments of fibronectin has a cell binding domain.

Application

Fibronectin Proteolytic Fragment from human plasma has been used in pre adsorbed antibody blocking assay and EC-pericyte tube coassembly.

Actions biochimiques/physiologiques

Fibronectin participates in cell adhesion, growth, migration, wound healing, blood coagulation and metastasis. Mutations in FN1 results in glomerulopathy. It plays an important role in cell attachment and spreading, control of cell cytoskeleton, morphology and differentiation. FN1 is also involved in extracellular matrix formation, hemostasis and thrombosis. Proteolytic fragments of fibronectin plays a vital role in mononuclear phagocyte function.

Attention

The product should be stored at -20°C.

Notes préparatoires

This product is lyophilized from phosphate buffered saline with sucrose as a cryoprotectant. The source material has tested negative for antibody to HIV, HCN, and HBsAg. It is soluble in water at 0.5 mg/mL and yields a clear to slightly hazy solution.

Pictogrammes

Exclamation mark
GHS07

Mention d'avertissement

Warning

Mentions de danger

H315,H319,H335

Classification des risques

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Organes cibles

Respiratory system

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Ail protein binds ninth type III fibronectin repeat (9FNIII) within central 120 kDa region of fibronectin to facilitate cell binding by Yersinia pestis.
Tsang T M, et al.
The Journal of Biological Chemistry, 287(20), 16759-16767 (2012)
Fibronectin in Health and Disease (2018)
C Penn et al.
Microbial pathogenesis, 17(6), 387-393 (1994-12-01)
Candida albicans yeast cells bind soluble human plasma fibronectin (Fn) through a glycoprotein receptor (adhesin) located on the cell surface. This work demonstrates that a 120 kDa proteolytic fragment of Fn encompassing the cell binding domain binds more avidly to
Pericyte recruitment during vasculogenic tube assembly stimulates endothelial basement membrane matrix formation.
Stratman A N, et al.
Blood, 114(24), 5091-5101 (2009)
Silke Niemann et al.
mBio, 12(5), e0106221-e0106221 (2021-10-20)
While Staphylococcus aureus has classically been considered an extracellular pathogen, these bacteria are also capable of being taken up by host cells, including nonprofessional phagocytes such as endothelial cells, epithelial cells, or osteoblasts. The intracellular S. aureus lifestyle contributes to
Afficher tous les articles scientifiques apparentés

Articles

Extracellular Matrix Proteins and Tools for Cell Culture Optimization

Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

Protocoles

Fibronectin Coating Protocol

Dilute fibronectin to the desired concentration. Optimum conditions for attachment are dependent on cell type and application. The typical coating concentration is 1 – 5 ug/cm2.Fibronectin coating protocol, products, and FAQs at sigmaaldrich.com

Fibronectin Coating Protocol

Dilute fibronectin to the desired concentration. Optimum conditions for attachment are dependent on cell type and application. The typical coating concentration is 1 – 5 ug/cm2.Fibronectin coating protocol, products, and FAQs.

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