172426

Glucoamylase, Rhizopus sp.

Glucoamylase is a multi-domain enzyme that belongs to the glycosyl hydrolase 15 family. Useful for the enzymatic determination of starch.

• Synonyme(s) : Glucoamylase, Rhizopus sp., Glucan 1,4-α-glucosidase, Glucoamylase, Amyloglucosidase, γ-Amylase, Lysosomal α-glucosidase, exo-4,7-α-glucosidase
• Numéro CAS: 9032-08-0
• Numéro de classification (Commission des enzymes): 3.2.1.3 (BRENDA, IUBMB)
• Numéro MDL: MFCD00081350
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.54

Propriétés

• Source biologique: fungus (Rhizopus spp.)
• Niveau de qualité: 100
• Forme: lyophilized
• Activité spécifique: ≥10 unit/mg solid; ≥20 units/mg protein
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze
• Solubilité: aqueous buffer: 1 mg/mL; water: 1 mg/mL
• Conditions d'expédition: wet ice
• Température de stockage: −20°C

Description

Description générale

Native glucoamylase from Rhizopus species. Glucoamylase is a multi-domain enzyme that belongs to the glycosyl hydrolase 15 family. It catalyzes the hydrolysis of 1,4-linked α-D-glucose residues in glucosylpolysaccharides, liberating glucose units. Useful for the enzymatic determination of starch.

Native glucoamylase from Rhizopus species. Glucoamylase is a multi-domain enzyme that belongs to the glycosyl hydrolase 15 family. It catalyzes the hydrolysis of 1,4-linked α-D-glucose residues in glucosylpolysaccharides, liberating glucose units. Useful for the enzymatic determination of starch.

Avertissement

Toxicity: Standard Handling (A)

Définition de l'unité

One unit is defined as the amount of enzyme that will liberate 1 µmol glucose from maltopentaose per min at 37°C, pH 6.0.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C. Avoid freeze/thaw cycles of solutions.

Autres remarques

Hiromi, K., et al. 1973. Biochim. Biophys. Acta302, 362.
Kelly, J.J. and Alpers, D.H. 1973. Biochim. Biophys. Acta315, 113.
Jeffrey, P.L., et al. 1970. Biochemistry9, 1403.
Brown, B. and Brown, D.H. 1965. Biochim. Biophys. Acta110, 124.
Tsujisaka, Y., et al. 1958. Nature181, 770.
Miller, K.D. and Copeland, W.H. 1956. Biochim. Biophys. Acta22, 193.
French, D. and Knapp, D.W. 1950. J. Biol. Chem.187, 463.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H334
• Conseils de prudence: P261 - P284 - P501
• Classification des risques: Resp. Sens. 1
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable