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S5133

Sigma-Aldrich

Schiff′s fuchsin-sulfite reagent

liquid

Synonym(s):

Schiff reagent

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About This Item

Empirical Formula (Hill Notation):
C20H21N3O3S
Molecular Weight:
383.46
UNSPSC Code:
12171500
NACRES:
NA.47

product name

Schiff′s fuchsin-sulfite reagent, suitable for detection of glycoproteins

Assay

3-5 mM iodine (I) basis (titration)

Quality Level

100

form

liquid

technique(s)

microbe id | staining: suitable

color

colorless

solubility

water: soluble

density

0.990 g/cm3

suitability

suitable for detection of glycoproteins

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

General description

Schiff’s fuchsin-sulfite reagent is a part of the glycoprotein detection kit. It is primarily used in the Periodic Acid-Schiff (PAS) staining method to detect and visualize aldehydes, glycoproteins in biological samples. The reagent consists of basic fuchsin dye and sodium bisulfite. It reacts with the aldehyde groups of carbohydrates and produces a pink to magenta color, allowing for the identification of these substances in samples.

Application

Schiff′s fuchsin-sulfite reagent has been used:

  • in periodic acid-Schiff (PAS) staining to determine goblet cells and salivary glycopreoteins like mucins
  • to stain ovaries and testis
  • to stain tomato and Medicago primary roots
  • to stain undifferentiated sarcomas (USARCs)
It provides a system to easily detect the sugar moieties of glycoproteins on SDS PAGE or Western Blotting membranes.

Preparation Note

Prepared using pararosaniline.

Pictograms

Health hazardCorrosion
GHS08,GHS05

Signal Word

Danger

Hazard Statements

H290,H350

Hazard Classifications

Carc. 1B - Met. Corr. 1

Storage Class Code

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
MKCN1525
MKCM1419
MKCH9605
MKCD9420
MKCB9884

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Identification of salivary proteins at oil-water interfaces stabilized by lysozyme and ?-lactoglobulin
Erika Silleti
Journal of Archaeology of Egypt/Egyptology (2010)
Undifferentiated Sarcomas Develop through Distinct Evolutionary Pathways
Steele CD, et al.
Cancer Cell, 35(3), 441-456 (2019)
Functionality of Soy Protein Produced
by Enzyme-Assisted Extraction
S Jung
J. Oil Colour Chem. Assoc. (2006)
The RNA m6A reader YTHDF2 is essential for the post-transcriptional regulation of the maternal transcriptome and oocyte competence
Ivanova I, et al.
Molecular Cell, 67(6), 1059-1067 (2017)
Crisalejandra Rivera-Perez et al.
Scientific reports, 10(1), 20201-20201 (2020-11-21)
Nacre is the main component of the pearl oyster shells and it is synthesized by specialized soluble and insoluble shell matrix proteins. Insoluble proteins from the decalcification of the shell are the less studied proteins due to the technical problems
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Articles

NMR Analysis of Glycans

Nuclear magnetic resonance (NMR) spectroscopy measures the extent to which a glycan or other molecule distorts a magnetic field.

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