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Merck

TOX6

Sigma-Aldrich

In Vitro Toxicology Assay Kit, Sulforhodamine B based

Sinónimos:

biomass viability assay, sulforhodamine B, total biomass assay

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About This Item

Código UNSPSC:
12352207
NACRES:
NA.84

Formulario

liquid

Nivel de calidad

uso

 kit sufficient for 1,000 tests

envase

pkg of 1 kit

condiciones de almacenamiento

dry at room temperature

λmáx.

565 nm

aplicaciones

cell analysis
detection

método de detección

colorimetric

temp. de almacenamiento

room temp

Descripción general

The Sulforhodamine B method is simple, accurate, and provides consistent results. This assay system is a means of measuring total biomass by staining cellular proteins with sulforhodamine B.

Aplicación

In Vitro Toxicology Assay Kit, Sulforhodamine B based has been used for spectrophotometric measurement of biomass (viable and non-viable cells) by total protein. It has also been used to study the effect of 17-allylamino-17-demethoxygeldanamycin (17-AAG) on proliferation of the five uveal melanoma cell lines. Absorbance of dye is measured at a wavelength of 565nm.

Acciones bioquímicas o fisiológicas

Dye binds to cellular protein and is then solubilized in base.

Palabra de señalización

Danger

Frases de peligro

Clasificaciones de peligro

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Flam. Liq. 3 - Skin Corr. 1A

Código de clase de almacenamiento

3 - Flammable liquids


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Immune expression and inhibition of heat shock protein 90 in uveal melanoma
Faingold D, et al.
Clinical Cancer Research, 14(3), 847-855 (2008)
Monocarboxylate transporters (MCTs) in gliomas: expression and exploitation as therapeutic targets
Miranda G, et al.
Neuro-Oncology, 15(2), 172-188 (2012)
P Skehan et al.
Journal of the National Cancer Institute, 82(13), 1107-1112 (1990-07-04)
We have developed a rapid, sensitive, and inexpensive method for measuring the cellular protein content of adherent and suspension cultures in 96-well microtiter plates. The method is suitable for ordinary laboratory purposes and for very large-scale applications, such as the
Jae-Hyeog Choi et al.
Oncoimmunology, 7(5), e1421890-e1421890 (2018-05-04)
Combination therapies with phosphoinositide 3-kinase (PI3K) inhibitors and trastuzumab (anti-human epidermal growth factor receptor [HER]2/neu antibody) are effective against HER2+ breast cancer. Isoform-selective PI3K inhibitors elicit anti-tumor immune responses that are distinct from those induced by inhibitors of class I
Marike Gabrielson et al.
PloS one, 9(9), e107109-e107109 (2014-09-23)
Epithelial ovarian carcinoma (EOC), the major cause of gynaecological cancer death, is a heterogeneous disease classified into five subtypes. Each subtype has distinct clinical characteristics and is associated with different genetic risk factors and molecular events, but all are treated

Artículos

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

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