TOX4
In Vitro Toxicology Assay Kit, Neutral Red based
Sinónimos:
NR uptake assay, Neutral red assay
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About This Item
Código UNSPSC:
12352207
NACRES:
NA.84
Productos recomendados
Formulario
liquid
Nivel de calidad
uso
1 kit sufficient for 1,000 tests
envase
pkg of 1 kit
condiciones de almacenamiento
dry at room temperature
λmáx.
540 nm
aplicaciones
cell analysis
detection
método de detección
colorimetric
temp. de almacenamiento
2-8°C
Descripción general
For spectrophotometric measurement of viable cells. Absorbance of converted dye is measured at a wavelength of 540 nm.
Aplicación
In Vitro Toxicology Assay Kit, Neutral Red based has been used:
- to measure the lysosomal enzyme activity in HB8902 cells
- to study the cell viability of human alveolar epithelial cells
- to study the cell viability in fibroblasts
Acciones bioquímicas o fisiológicas
Neutral red (NR) is a red dye that accumulates in the lysosome by actively transferring across the cell membranes. The dye is extracted and the uptake is quantitated by spectroscopy. The NR uptake assay is the most commonly used cytotoxicity test. It is widely applicable in environmental and biomedical studies to estimate the number of viable cells in culture. The NR dye in vitro tissue culture studies is used in assessing viral cytopathogenicity and detection of toxic compounds.
Palabra de señalización
Danger
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Carc. 1B - Flam. Liq. 3 - Skin Sens. 1
Código de clase de almacenamiento
3 - Flammable liquids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
73.4 °F
Punto de inflamabilidad (°C)
23 °C
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In vitro cellular & developmental biology : journal of the Tissue Culture Association, 27A(3 Pt 1), 239-244 (1991-03-01)
A new three-dimensional human skin model consisting of several layers of actively dividing and metabolically active human neonatal foreskin-derived fibroblasts and epidermal keratinocytes grown on nylon mesh has been used to assess the in vitro toxicity of test agents from
Guillermo Repetto et al.
Nature protocols, 3(7), 1125-1131 (2008-07-05)
The neutral red uptake assay provides a quantitative estimation of the number of viable cells in a culture. It is one of the most used cytotoxicity tests with many biomedical and environmental applications. It is based on the ability of
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The neutral red (NR) absorption method was adapted for the determination of cell viability in the UDS assay with primary hepatocyte cultures of the rat. The NR method is rapid, easy to perform, and suitable for handling of large numbers
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