T2949

Anti-mTOR antibody produced in rabbit

1.0-1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

• Sinónimos: Anti-FKBP-12 rapamycin associated protein, Anti-FRAP, Anti-RAFT, Anti-SEP
• Número MDL: MFCD02263996
• Código UNSPSC: 12352203

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 200
• conjugado: unconjugated
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• formulario: buffered aqueous solution
• mol peso: antigen 289 kDa
• reactividad de especies: rat, human, mouse
• concentración: 1.0-1.5 mg/mL
• técnicas: immunoprecipitation (IP): 1.0-1.5 μg using 300 μg RIPA extract of human transformed kidney HEK 293T cells; microarray: suitable; western blot: 1:2,000 using whole extracts of rat brain tissue; western blot: 1:500 using mouse brain tissue
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... FRAP1(2475) ; mouse ... Frap1(56717) ; rat ... Frap1(56718)

Descripción

Descripción general

mTOR is an evolutionary conserved serine/threonine kinase that belongs to the phosphoinositol kinase-related kinase (PIKK) family. This kinase regulates cell growth by initiation of translation in response to nutrients such as amino acids (primarily leucine), growth factors, mitogens and insulin. Translational initiation is mediated by mTOR through S6K1 activation and 4E-BP1 inhibition. mTOR may also modulate several cellular functions such as actin organization, secretion, membrane trafficking, protein degradation, protein kinase C signaling, tRNA synthesis and ribosome biogenesis. Anti-mTOR antibody is specific for human, mouse and rat mTOR (289 kDa).

Especificidad

Anti-mTOR (FRAP) is produced using a peptide corresponding to amino acids 2433-2450 of human mTOR. The corresponding sequence is identical in mouse and differs by one amino acid in rat. The antibody specifically recognizes human, mouse and rat mTOR (289 kDa) and is suitable for immunoblotting, immunoprecipitation and immunofluorescence.

Inmunógeno

The corresponding sequence is identical in mouse and differs by one amino acid in rat.

synthetic peptide corresponding to amino acid residues 2433-2450 of human mTOR.

Aplicación

Anti-mTOR antibody is suitable for use in Western blot (1:1,000 using myotube cell lysates of rainbow trout cultures). The antibody can also be used in indirect immunofluorescence, immunoprecipitation, and microarray.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable