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Merck

M5670

Sigma-Aldrich

Anti-MAP Kinase (ERK-1, ERK-2) antibody produced in rabbit

whole antiserum

Sinónimos:

Anti-MAP Kinase (ERK-1, ERK-2), Anti-Mitogen Activated Protein Kinase

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About This Item

Número MDL:
Código UNSPSC:
12352203
NACRES:
NA.44

origen biológico

rabbit

Nivel de calidad

conjugado

unconjugated

forma del anticuerpo

whole antiserum

tipo de anticuerpo

primary antibodies

clon

polyclonal

mol peso

antigen ERK-2 42 kDa
antigen, ERK-1 44 kDa

contiene

15 mM sodium azide

reactividad de especies

human, rat, mouse

envase

antibody small pack of 25 μL

técnicas

microarray: suitable
western blot: 1:40,000-1:20,000 using rat brain extract and mouse NIH 3T3 fibroblast extract, respectively

Nº de acceso UniProt

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

unmodified

Información sobre el gen

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Descripción general

MAP kinase (MAPK, mitogen-activated protein kinase) is also termed as extracellular regulated protein kinase (ERK). It consists of a family of protein kinases. MAP kinase isoforms are widely expressed in the central nervous system, thymus, spleen, heart, lung, kidney, and in high levels in PC12 cells and in fibroblasts.

Especificidad

Staining of the 42 kDa and 44 kDa bands is specifically inhibited with MAP kinase peptide (317-339), but not with MAP kinase kinase (MEK) peptide (34-48) corresponding to N-terminal sequence of MEK. Anti-MAPK Kinase (ERK-1, ERK-2) specifically reacts with both phosphorylated and unphosphorylated forms of ERK-1 and ERK-2 (44 and 42 kDa, respectively).

Inmunógeno

synthetic peptide corresponding to amino acids 317-339 derived from subdomain XI of human MAP kinase (ERK1).

Aplicación

Anti-MAP Kinase (ERK-1, ERK-2) antibody produced in rabbit has been used in western blot analysis at a minimum dilution of 1:40000 in rat brain extract and at 1:20000 in mouse NIH 3T3 fibroblasts.

Acciones bioquímicas o fisiológicas

Mitogen-activated protein kinase (MAPK) superfamily of enzymes is involved in widespread signalling pathways. Members of this family include the ERK1/2 (extracellular signal-regulated protein kinase, also termed p42/p44 MAPK), JNK and p38 MAPK subfamilies. These are the terminal enzymes in a signalling cascade where each kinase phosphorylates and activates the next member in the sequence. Phosphorylation of both tyrosine and threonine is essential for the full activation of all MAPKs. Several kinases participate in activation of the ERK cascade. This cascade is initiated by the small G protein Ras, which upon stimulation causes activation Raf1 kinase. Raf1 continues the transmission by activating MEK. Activated MEK appears to be the only kinase capable of specifically phosphorylating and activating ERK. ERK appears to be an important regulatory molecule, which by can phosphorylate regulatory targets in the cytosol (phospholipase A2, PLA2), translocated into and phosphorylate substrates in the nucleus (ELK1). The activation of ERK cascade mediates and regulates the signal transduction pathways in response to stress, mitogenic signals and is important in development and differentiation, learning, memory and survival. MAPK plays a crucial role in various signal transduction pathways, leading signals of growth factor and G protein coupled receptors to their intracellular targets. MAP kinase regulates several cellular processes including proliferation, differentiatio, cellular morphology and oncogenesis.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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