T0157

Tumor Necrosis Factor-α human

TNF-α, recombinant, expressed in yeast, buffered aqueous solution, suitable for cell culture

• Sinónimos: hTNF-α, TNF-α
• Número de CAS: 94948-59-1
• Número MDL: MFCD00148692
• Código UNSPSC: 12352202
• NACRES: NA.77

Propiedades

• origen biológico: human
• recombinante: expressed in yeast
• Análisis: ≥95% (SDS-PAGE)
• formulario: buffered aqueous solution
• potencia: >1x108 units/mg ED₅₀
• calidad: endotoxin tested
• actividad específica: ≥2 × 10⁷ U/mg
• mol peso: ~17.4 kDa
• envase: pkg of 10 μg
• condiciones de almacenamiento: avoid repeated freeze/thaw cycles
• concentración: 0.1-10 mg/mL
• técnicas: cell culture | mammalian: suitable
• impurezas: <10 EU/mLtested (LAL test)
• color: colorless
• Nº de acceso UniProt: P01375
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• Información sobre el gen: human ... TNF(7124)

Descripción

Descripción general

TNFα (tumor necrosis factor α) belongs to the TNF superfamily and is mapped to human chromosome 6p21.33.

Aplicación

Tumor Necrosis Factor-α human has been used to study the differential regulation of microRNA-146a , CFH (complement factor-H), IRAK-1 (interleukin-1 receptor associated kinase-1) and tetraspanin-12 in primary brain cell cultures in presence of TNF-α. To determine the intercellular adhesion molecule-1 (ICAM1) expression using ELISA.

Acciones bioquímicas o fisiológicas

TNF-α is associated with formation, growth and destabilization of atherosclerotic plaques. TNF-α is involved in the disease progression of osteoarthritis.

Tumore necrosis factor-α (TNF-α), also known as cachectin, is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-α converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. TNF-α plays roles in antitumor activity, immune modulation, inflammation, anorexia, cachexia, septic shock, viral replication and hematopoiesis. TNF-α is expressed by a great variety of cells, with numerous inductive and suppressive agents. Primarily, TNF-α is produced by macrophages in response to immunological challenges such as bacteria (lipopolysaccharides), viruses, parasites, mitogens and other cytokines. TNF-α is cytotoxic for many transformed cells (its namesake activity) but in normal diploid cells, it can stimulate proliferation (fibroblasts), differentiation (myeloid cells) or activation (neutrophils). TNF-α also shows antiviral effects against both DNA and RNA viruses and it induces production of several other cytokines. Although TNF-α is used in clinical trials as an antitumor agent, Sigma′s cytokine, growth factor and hormone products are for research only. TNF-α and the related molecule TNF-β (LT-α) share close structural homology with 28% amino acid sequence identity and both activate the same TNF receptors, TNFR1 and TNFR2. Mouse and human TNF-α share 79% amino acid sequence identity. Unlike human TNF-α, the mouse form is N-glycosylated.

Forma física

Solution in phosphate buffered saline containing 1 mg/ml bovine serum albumin.

Nota de análisis

The cytolytic activity of TNF-α against WEHI 164 cells has been measured in culture using an MTT toxicity assay. The ED50 is defined as the concentration of TNF-alpha that mediates half-maximal cytotoxicity in the presence of 1 micro-g/ml actinomycin D.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)