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Merck

SRP9001

Sigma-Aldrich

Leukemia Inhibitory Factor human

≥95% (SDS-PAGE), recombinant, expressed in HEK 293 cells, liquid, suitable for cell culture

Sinónimos:

Differentiation-stimulating factor, Emfilermin, LIF, Melanoma-derived LPL inhibitor

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About This Item

Código UNSPSC:
12352202
NACRES:
NA.32

product name

Leukemia Inhibitory Factor, human, recombinant, expressed in HEK 293 cells

origen biológico

human

Nivel de calidad

recombinante

expressed in HEK 293 cells

Análisis

≥95% (SDS-PAGE)

formulario

liquid

potencia

≤0.5 ng/mL M1 cells proliferation inhibition EC50

mol peso

20 kDa

envase

pkg of 10 μg

técnicas

cell culture | mammalian: suitable

impurezas

≤1 EU/μg protein Endotoxin level

Nº de acceso UniProt

temp. de almacenamiento

−20°C

Información sobre el gen

human ... LIF(3976)

Descripción general

LIF is manufactured using an all-human production system, with no serum.
Leukemia Inhibitory Factor (LIF) gene is mapped on the human chromosome at 22q12.2.
Leukemia inhibitory factor (LIF) is a secreted pleiotropic glycoprotein which is a member of the gp130 signaling of interleukin-6 (IL-6) family cytokines. This protein was originally identified as a protein that prevented the growth of a leukemic cell line.

Aplicación

Leukemia inhibitory factor (LIF) has been used to evaluate its effect on embryo development and aneuploidy in matured (IVM) human oocytes, in vitro.
One of the main uses of LIF in research is in mouse embryonic stem cell culture maintenance. LIF is necessary to maintain the stem cells in an undifferentiated state while retaining their proliferative potential or pluripotency. Removal of LIF pushes stem cells toward differentiation.

Acciones bioquímicas o fisiológicas

Leukemia inhibitory factor (LIF) suppresses the differentiation of myoblasts. It also inhibits the activation of caspase-3 and blocks the reduction in Bcl-xL (B cell lymphoma) levels, thus, preventing doxorubicin-induced apoptosis. Along with transforming growth factor β2 (TGFβ2)/fibroblast growth factor 2 (FGF2), LIF regulates nephrogenesis. It facilitates tubular regeneration following acute kidney failure. It plays a crucial role in neuroprotective pathways induced by preconditioning-induced oxidative stress. LIF is capable of maintaining the totipotency of embryonic stem cells. It is also involved in controlling the differentiation of embryonic stem cells, hematopoietic and neuronal cells.

Forma física

This product is supplied as a solution in 0.2 μm filtered phosphate buffered saline with no additives or carrier proteins. It is aseptically filled.

Nota de preparación

Briefly centrifuge the vial before opening. After initial thawing it is recommended to store the protein in working aliquots at -20°C. The product can be diluted in PBS.

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Protective effects of leukemia inhibitory factor against oxidative stress during high glucose-induced apoptosis in podocytes.
Xu J et al
Cell Stress & Chaperones, 17(4), 485-493 (2012)
Cytokines hold promise for human embryo culture in vitro: results of a randomized clinical trial
Fawzy M, et al.
Fertility and Sterility, 112(5), 849-857 (2019)
Leukemia inhibitory factor (LIF) and its biological activity.
Chodorowska G et al
Annales Universitatis Mariae Curie-Sklodowska. Sectio D: Medicina, 59(2), 189-193 (2004)
The gp130 receptor cytokine family: regulators of adipocyte development and function.
White UA and Stephens JM
Current Pharmaceutical Design, 17(4), 340-346 (2011)
Complex conserved organization of the mammalian leukemia inhibitory factor gene: regulated expression of intracellular and extracellular cytokines.
Haines BP et al
Journal of Immunology, 162(8), 4637-4646 (1999)

Artículos

Read article on hematopoietic cytokines and hematopoiesis

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