SCU0001

Interleukin-6 human

recombinant, expressed in HEK 293 cells, suitable for cell culture

• Sinónimos: IL-6
• Número MDL: MFCD00131403
• Código UNSPSC: 12352202
• NACRES: NA.77

Propiedades

• origen biológico: human
• Nivel de calidad: 200
• recombinante: expressed in HEK 293 cells
• Análisis: ≥98% (SDS-PAGE)
• formulario: lyophilized powder
• potencia: 0.2-2 ng/mL EC₅₀
• calidad: endotoxin tested
• mol peso: calculated mol wt 20.8 kDa (183 amino acids glycosylated protein)
• envase: vial of ≥10 μg
• técnicas: cell culture | mammalian: suitable
• impurezas: ≤0.1 EU/μg protein (LAL test)
• color: white
• Nº de acceso UniProt: P05231
• temp. de almacenamiento: −20°C
• Información sobre el gen: human ... IL6(3569)

Descripción

Descripción general

Interleukin 6 (IL-6) is a pleiotropic glycoprotein belonging to the IL-6 family of cytokines. IL-6 is a multifunctional cytokine with well-defined pro- and anti-inflammatory properties. IL-6 is secreted by T cells and macrophages to stimulate immune response, e.g. during infection and after trauma, especially burns or other tissue damage leading to inflammation. The O-linked sugar chain of IL-6 is thought to contribute to the stability of the cytokine through polymerization suppression and/or conformational changes, exemplifying the significance of its expression in a human expression system (which allows proper glycosylation).

Acciones bioquímicas o fisiológicas

Interleukin-6 (IL-6) is a multifunctional protein originally discovered in the media of cells stimulated with double stranded RNA. IL-6 appears to be directly involved in the responses that occur after infection and injury and may prove to be as important as IL-1 and TNF-α in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages, and endothelial cells. It acts upon a variety of cells, including fibroblasts, myeloid progenitor cells, T cells, B cells and hepatocytes. IL-6 induces multiple effects, as indicated by its numerous synonyms: plasmacytoma growth factor (PCT-GF), interferon-β-2 (IFN-β₂), monocyte derived human B cell growth factor, B cell stimulating factor (BSF-2), hepatocyte stimulating factor (HSF), Interleukin Hybridoma/Plasmacytoma-1 (IL-HP1). In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 also potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors.

Forma física

This product is supplied as a lyophilized powder in phosphate buffered saline (PBS), pH 7.4, with no additives or carrier proteins. It is aseptically filled.

Nota de preparación

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile water to a final concentration of 100 μg/ml. Adding 0.1% recombinant human serum albumin is recommended. The product is stable for at least 2 years as supplied. After reconstitution, it is recommended to store the protein in working aliquots at –20°C.

Nota de análisis

The biological activity of recombinant human IL-6 was tested in culture by measuring its ability to stimulate proliferation of the IL-6 dependent mouse T1165 cells.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable