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Merck

L5158

Sigma-Aldrich

Leukemia Inhibitory Factor from mouse

≥95% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous solution, suitable for cell culture

Sinónimos:

LIF

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About This Item

Número MDL:
Código UNSPSC:
12352202
NACRES:
NA.77

Nombre del producto

Leukemia Inhibitory Factor from mouse, LIF, recombinant, expressed in E. coli, 10 μg/ml, buffered aqueous solution, suitable for cell culture

recombinante

expressed in E. coli

Nivel de calidad

Ensayo

≥95% (SDS-PAGE)

Formulario

buffered aqueous solution

calidad

endotoxin tested

técnicas

cell culture | mammalian: suitable

temp. de almacenamiento

2-8°C

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Aplicación

Leukemia Inhibitory Factor from mouse has been used:
  • as a component of enriched co-culture media
  • as a supplement in Dulbecco′s modified eagle′s medium (DMEM)/F12 medium for culturing heart cells
  • as a component in maintenance medium for maintaining embryonic stem cells
  • to treat ovaries in ovarian culture

Acciones bioquímicas o fisiológicas

Leukemia Inhibitory Factor (LIF) is a pleiotropic glycoprotein originally described to inhibit the proliferation of the murine myeloid leukemic cell line M1, while inducing differentiation into macrophages. Other activities were later identified to LIF, which is known by a variety of synonyms, including DIF, D-factor, DIA, DRF, CNDF, HILDA, HSF-III, and MLPLI. Human LIF exerts its actions through a receptor comprising a 190 kDa LIF-binding α-chain (130 kDa, mouse) and a 130 kDa signal-transducing β-chain (gp130), which is shared with CNTF, OSM, L-6 and IL-11. As such, it is a member of the gp130 family of the cytokine receptor superfamily. LIF receptors have been identified on several cells, including monocytes, liver, placenta and embryonic stem cells. Natural LIF is heavily glycosylated, showing an apparent molecular weight of 32 kDa to 62 kDa, depending on the source, but absence of glycosylation appears not to affect its biological activity. A single gene encodes LIF, which is secreted as a single chain glycoprotein containing 180 amino acids for human or mouse with a conserved disulfide bond. Human and mouse LIF share 78% sequence homology. Human LIF can activate mouse cells, but mouse LIF cannot activate human cells.
Leukemia Inhibitory Factor (LIF) is a pleiotropic glycoprotein that inhibits the proliferation of the murine myeloid leukemic cell line M1, while inducing differentiation into macrophages. Human and mouse LIF share 78% sequence homology. Human LIF can activate mouse cells, but mouse LIF cannot activate human cells.

Forma física

Solution in phosphate buffered saline containing 0.02% TWEEN® 20

Nota de análisis

The proliferative activity of mouse LIF is measured in culture using the M1 murine cell line.

Información legal

TWEEN is a registered trademark of Croda International PLC

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)


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LIF-immobilized nonwoven polyester fabrics for cultivation of murine embryonic stem cells
Cetinkaya G, et al.
Journal of Biomedical Materials Research Part A, 81(4), 911-919 (2007)
C-Kit Positive Cardiac Stem Cells and Bone Marrow-Derived Mesenchymal Stem Cells Synergistically Enhance Angiogenesis and Improve Cardiac Function After Myocardial Infarction in a Paracrine Manner
Bao L, et al.
Journal of Cardiac Failure, 23(5), 403-415 (2017)
Renée F de Pooter et al.
Methods in molecular biology (Clifton, N.J.), 290, 135-147 (2004-09-14)
Lymphocytes arise during ontogeny via a series of increasingly restricted intermediates. Initially, the mesoderm gives rise to hemangioblasts, which can differentiate into endothelial precursors, or hematopoietic stem cells (HSCs). HSCs can either self-renew or differentiate into lineage-restricted progenitors and, ultimately
Suppressor of cytokine signaling 4 (SOCS4): moderator of ovarian primordial follicle activation
Sutherland JM, et al.
Journal of Cellular Physiology, 227(3), 1188-1198 (2012)
D P Gearing et al.
The EMBO journal, 6(13), 3995-4002 (1987-12-20)
Leukaemia inhibitory factor (LIF) can induce macrophage differentiation in M1 murine myeloid leukaemic cells and suppress their proliferation in vitro. It does not stimulate the proliferation of normal progenitor cells and is apparently distinct from known colony-stimulating factors. We have

Artículos

Leukemia Inhibitory Factor (LIF) is a stem cell growth factor used for the in vitro culture of pluripotent mouse embryonic stem cells (ES cells).

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