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Key Documents

SRP3200

Sigma-Aldrich

GDNF from mouse

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Sinónimos:

ATF-1, Glial-Derived Neurotrophic Factor

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About This Item

Código UNSPSC:
12352202
NACRES:
NA.32

origen biológico

mouse

recombinante

expressed in E. coli

Análisis

≥98% (HPLC)
≥98% (SDS-PAGE)

formulario

lyophilized

potencia

≤0.2 ng/mL

mol peso

15.1 kDa

envase

pkg of 10 μg

técnicas

cell culture | mammalian: suitable

impurezas

<0.1 EU/μg endotoxin, tested

color

white to off-white

Nº de acceso UniProt

Condiciones de envío

wet ice

temp. de almacenamiento

−20°C

Información sobre el gen

mouse ... GDNF(14573)

Descripción general

GDNF (glial cell line derived neurotrophic factor) is a disulfide-linked homodimeric neurotrophic factor structurally related to Artemin, Neurturin and Persephin. These proteins belong to the cysteine-knot superfamily of growth factors that assume stable dimeric protein structures. The functional murine GDNF ligand is a disulfide-linked homodimer, of two 15.1kDa polypeptide chains called monomers. Each monomer contains seven conserved cysteine residues, one of which is used for inter-chain disulfide bridging and the others are involved in intramolecular ring formation known as the cysteine knot configuration.

Aplicación

GDNF from mouse has been used for stimulating primary murine cultures of dorsal root ganglion (DRG) neurons.
GDNF from mouse has been used to study the role of NEDL2 (Nedd4-related E3 ubiquitin ligase-2) in GDNF/Ret signaling.

Acciones bioquímicas o fisiológicas

GDNF (glial cell line derived neurotrophic factor) signals through a multicomponent receptor system, composed of a RET (receptor tyrosine kinase) and one of the four GFR-α (α1-α4) receptors (GDNF family receptors). GDNF specifically promotes dopamine uptake and survival and morphological differentiation of midbrain neurons. Using Parkinson′s disease monkey model, GDNF has been shown to improve conditions such as bradykinesia, rigidity, and postural instability. It might also be involved in the treatment of non-alcoholic fatty liver disease. GDNF is needed for proper spermatogonial stem cell survival and proliferation.

Secuencia

MSPDKQAAAL PRRERNRQAA AASPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCESAETMY DKILKNLSRS RRLTSDKVGQ ACCRPVAFDD DLSFLDDNLV YHILRKHSAK RCGCI

Forma física

Lyophilized from 10 mM Sodium Citrate.

Reconstitución

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Visite la Librería de documentos

Peripheral expression and biological activities of GDNF, a new neurotrophic factor for avian and mammalian peripheral neurons.
Trupp M, et al.
The Journal of Cell Biology, 130, 137-148 (1995)
Functional recovery in parkinsonian monkeys treated with GDNF.
Gash DM, et al.
Nature, 380, 252-255 (1996)
A rapid induction mechanism for Lin28a in trophic responses
Amen Am, et al.
Molecular Cell, 65(3), 490-503 (2017)
Peritubular myoid cells participate in male mouse spermatogonial stem cell maintenance.
Chen LY, et al.
Endocrinology, 155, 4964-4974 (2014)
NEDL2 is an essential regulator of enteric neural development and GDNF/Ret signaling.
Wei R, et al.
Cellular Signalling, 27, 578-586 (2015)

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