SRP0258

PDE1B Active human

recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)

• Sinónimos: Calcium/calmodulin-stimulated cyclic nucleotide phosphodiesterase, Cam-PDE 63 kDa, PDES1B, Phosphodiesterase 1B
• Código UNSPSC: 12352200
• NACRES: NA.32

Propiedades

• origen biológico: human
• recombinante: expressed in baculovirus infected insect cells
• Ensayo: ≥70% (SDS-PAGE)
• Formulario: aqueous solution
• actividad específica: ≥2000 units/μg
• mol peso: 87 kDa
• envase: pkg of 10 μg
• concentración: >0.02 mg/mL
• Nº de acceso NCBI: NM_000924
• Nº de acceso UniProt: Q01064
• Condiciones de envío: dry ice
• temp. de almacenamiento: −70°C
• Información sobre el gen: human ... PDE1B(5153)

Descripción

Descripción general

Human PDE1B (GenBank Accession No. NM_000924), full length with N-terminal GST tag, MW = 87 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Aplicación

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Definición de unidad

One unit is defined as the amount of enzyme that will convert 1 pmole of 3′, 5′-cAMP to 5′ AMP per min at 37°C

Forma física

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol and 3 mM DTT.

Nota de preparación

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.