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Merck

SML1633

Sigma-Aldrich

7-AAD

≥97% (HPLC), liquid (solution in DMSO:H2O (1:1)), fluorescent indicator

Sinónimos:

7-Aminoactinomycin D Ready Made Solution, 7-Aminodactinomycin Ready Made Solution

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About This Item

Fórmula empírica (notación de Hill):
C62H87N13O16
Número de CAS:
Peso molecular:
1270.43
Código UNSPSC:
12352200
NACRES:
NA.77

product name

7-AAD Ready Made Solution, 1 mg/mL

formulario

liquid (solution in DMSO:H2O (1:1))

Nivel de calidad

condiciones de almacenamiento

protect from light

concentración

1 mg/mL

temp. de almacenamiento

−20°C

InChI

1S/C62H87N13O16/c1-26(2)42-59(85)74-21-17-19-36(74)57(83)70(13)24-38(76)72(15)48(28(5)6)61(87)89-32(11)44(55(81)66-42)68-53(79)34-23-35(63)30(9)51-46(34)65-47-40(41(64)50(78)31(10)52(47)91-51)54(80)69-45-33(12)90-62(88)49(29(7)8)73(16)39(77)25-71(14)58(84)37-20-18-22-75(37)60(86)43(27(3)4)67-56(45)82/h23,26-29,32-33,36-37,42-45,48-49H,17-22,24-25,63-64H2,1-16H3,(H,66,81)(H,67,82)(H,68,79)(H,69,80)

Clave InChI

YXHLJMWYDTXDHS-UHFFFAOYSA-N

Descripción general

Free form:
Excitation: 503 nm (0.01 M phosphate buffer, pH 7.0 containing 0.1 mM EDTA); 550 nm
Emission: 675 nm (0.01 M phosphate buffer, pH 7.0 containing 0.1 mM EDTA); 672 nm
DNA Complex:
Excitation: 543 nm; 555 nm
Emission: 655 nm; 665 nm

Aplicación

Fluorescent DNA stain. A featured product for Apoptosis research.

Acciones bioquímicas o fisiológicas

7-AAD is used in flow cytometry analysis of viable cells. 7-ADD is a fluorescent indicator that undergoes a spectral shift upon association with DNA. Su, X., et al. used FITC and PE-conjugated antibodies to stain cell surface markers. After cell-surface marker staining, cells were further stained with 10 μg/ml of 7-AAD in PBS on ice for 30 minutes. After washing with PBS twice, the cells were fixed in 1% paraformaldehyde supplemented with 50 μg/ml actinomycin D. Non-apoptotic cells were 7-AAD negative. 7-AAD like its parent molecule, Actinomycin D, is a DNA-intercalator with growth-inhibitory properties.
7-amino-actinomycin D (7-AAD) is a DNA dye. It can be used as a replacement for propidium iodide (PI) for the discrimination of dead cells. This dye is ideal for dead cell discrimination in long-lasting experiments, as it is efficiently exempted by intact cells and has a high DNA binding constant.

Reconstitución

Solution of 1 mg/ml in DMSO:H2O (1:1) is stable for at least 2 years at −20 °C without loss of activity. According to Su et al., the provided solution should be diluted to 10 μg/ml (1:100) working concentration in PBS and is therefore sufficient for 100 stain reactions.

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Dead cell discrimination with 7?amino?actinomcin D in combination with dual color immunofluorescence in single laser flow cytometry.
Schmid I, et al.
Cytometry. Part A : the Journal of the International Society For Analytical Cytology, 13(2), 204-208 (1992)
Jinwoo Seong et al.
Development (Cambridge, England), 145(14) (2018-06-29)
Mammary glands develop through primary ductal elongation and side branching to maximize the spatial area. Although primary ducts are generated by bifurcation of terminal end buds, the mechanism through which side branching occurs is still largely unclear. Here, we show

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