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646563

Supelco

DL-Ditiotreitol solution

1 M in H2O

Sinónimos:

(±)-treo-1,4-Dimercapto-2,3-butanediol solution, DTT

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About This Item

Fórmula empírica (notación de Hill):
C4H10O2S2
Número de CAS:
3483-12-3
Peso molecular:
154.25
Beilstein:
1719757
Número MDL:
MFCD00004877
Código UNSPSC:
12161504
ID de la sustancia en PubChem:
24883472
NACRES:
NA.25

formulario

liquid

Nivel de calidad

100

idoneidad de la reacción

reagent type: reductant

concentración

1 M in H2O

densidad

1.04 g/mL at 20 °C
1.035 g/mL at 25 °C

cadena SMILES

O[C@H](CS)[C@H](O)CS

InChI

1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1

Clave InChI

VHJLVAABSRFDPM-QWWZWVQMSA-N

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Descripción general

Dithiothreitol (DTT) is used in proteomics applications to maintain sulfhydryl (-SH) groups in the reduced state and for quantitative reduction of disulfide (-S-S-) groups. It is a frequently used reagent in buffers due to its ability to reduce the oxidation state of a protein sample and preserves enzymatic activity. DTT is oxidized to the cyclic disulfide during the reduction of other disulfides in the solution.
Dithiothreitol is an effective reducing agent for protein analysis. The sealed-ampule format is convenient and easy to use when preparing samples for electrophoresis or other applications and avoids waste and loss of potency.

Aplicación

DL-Dithiothreitol solution has been used:
  • in western blot analysis
  • as a supplement in lysis buffer to study its effects on sperm lysis
  • as a component of cold homogenization buffer, and chilled wash buffer for the isolation of nuclei from frozen rat tissues
  • in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), chromatography, modification of cysteine-containing compounds

Características y beneficios

Low toxicity and reduced odor compared to other thiol compounds.

Para utilizar con

Referencia del producto
Descripción
Precios

Pictogramas

Corrosion
GHS05

Palabra de señalización

Danger

Frases de peligro

H315,H318

Clasificaciones de peligro

Eye Dam. 1 - Skin Irrit. 2

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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L-(−)-Dithiothreitol ≥95% (titration)

Sigma-Aldrich

D9760

L-(−)-Dithiothreitol

Yodoacetamida Single use vial of 56 mg

Sigma-Aldrich

A3221

Yodoacetamida

Sputolysin® Reagent Intended for the isolation of epithelial cells, pathogenic or saprophytic bacteria, fungi, and yeasts from sputum.

Millipore

560000

Sputolysin® Reagent

DTT OmniPur® Grade, >= 99.4%, Used for reduction of protein disulfide linkages

Millipore

3860-OP

DTT

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In this study, we investigated the role of inositol polyphosphate-4-phosphatase, type-II (INPP4B) in acute myeloid leukemia (AML). We observed that AML patients with high levels of INPP4B (INPP4B(high)) had poor response to induction therapy, shorter event-free survival and shorter overall
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Pathogen effector proteins are delivered to host cells to suppress plant immunity. However, the mechanisms by which effector proteins function are largely unknown. Here we show that expression of XopP(Xoo), an effector of rice pathogen Xanthomonas oryzae pv. oryzae, in
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The EMBO journal, 34(13), 1801-1815 (2015-05-17)
Terminal uridylyl transferases (TUTs) function as integral regulators of microRNA (miRNA) biogenesis. Using biochemistry, single-molecule, and deep sequencing techniques, we here investigate the mechanism by which human TUT7 (also known as ZCCHC6) recognizes and uridylates precursor miRNAs (pre-miRNAs) in the
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