560000

Sputolysin^® Reagent

Intended for the isolation of epithelial cells, pathogenic or saprophytic bacteria, fungi, and yeasts from sputum.

• Número de CAS: 3483-12-3
• Número MDL: MFCD00004877
• Código UNSPSC: 12161703
• NACRES: NA.25

Propiedades

• Nivel de calidad: 100
• descripción: RTECS - EK1610000
• Formulario: liquid
• idoneidad de la reacción: reagent type: reductant
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze; protect from light
• Condiciones de envío: ambient
• temp. de almacenamiento: 15-25°C
• cadena SMILES: SC[C@H](O)[C@@H](O)CS
• InChI: 1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m0/s1
• Clave InChI: VHJLVAABSRFDPM-IMJSIDKUSA-N

Descripción

Descripción general

A concentrate of dithiothreitol (DTT) in phosphate buffer. Useful for the isolation of pathogenic or saprophytic bacteria, fungi, yeast, and epithelial cells from sputum and other mucoid fluids.

Concentrate of Dithiothreitol (DTT; Cat. No. 233155) in phosphate buffer, pH 6.5-7.5. Intended for the isolation of epithelial cells, pathogenic or saprophytic bacteria, fungi, and yeasts from sputum. Each vial is sufficient to make 100 ml of working solution.

Note: 1 set = 20 x 10 ml.

Advertencia

Toxicity: Harmful (C)

Forma física

20 vials each containing 10 ml of concentrated DTT OR 1 vial containing 10 ml of concentrated DTT.

Nota de preparación

Dilute the contents of one vial to 100 ml with sterile distilled water. Swirl gently to dissolve any crystals. If the diluted material appears cloudy, the solution may be filtered to remove any insoluble particles. The diluted solution is ~6.5 mM dithiothreitol in 100 mM phosphate buffer, pH 7.0.

Reconstitución

Once a vial is opened the unused concentrate should be purged with an inert gas (e.g. N₂) and refrigerated (4°C). Diluted solutions, stored aseptically under an inert gas, are stable for up to 48 h at 4°C.

Otras notas

Hirsch, S.R., et al. 1969. J. Lab. Clin. Med.74, 346.
Shah, R.R. and Dye, W.E. 1966. Am. Rev. Respir. Dis.94, 454.
Dixon, J.M.S. and Miller, D.C. 1965 (November). Lancet p. 1046.
Cleland, W.W. 1964. Biochemistry3, 480.
May, J.R. 1953 (September). Lancet p. 535.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

SPUTOLYSIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Pictogramas: GHS07
• Palabra de señalización: Warning
• Frases de peligro: H319
• Consejos de prudencia: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Clasificaciones de peligro: Eye Irrit. 2
• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable