SEQR
SeqPlex RNA Amplification Kit
For use with high throughput sequencing technologies
Sinónimos:
WGA kit, Whole transcriptome amplification
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About This Item
Productos recomendados
Nivel de calidad
técnicas
whole genome amplification: suitable
Condiciones de envío
wet ice
temp. de almacenamiento
−20°C
Categorías relacionadas
Descripción general
The SeqPlex™ RNA Amplification Kit for whole transcriptome amplification (WTA) is designed to facilitate next-generation sequencing (NGS) from small quantities or from degraded/highly fragmented RNA (e.g. RNA from formalin-fixed paraffin-embedded (FFPE) tissue samples). The SeqPlex kit allows the user to pre-amplify these and other small quantity/highly fragmented RNA samples for input into a NGS workflow. It also facilitates the amplification of non-polyA tailed RNA isolated from tissue, cultured cells, formalin-fixed samples, or serum while maintaining patterns of differential expression found in the unamplified sample.
Aplicación
SeqPlex™ RNA Amplification Kit has been used to amplify poly(A)-selected RNA.
Características y beneficios
- Low quantities of total RNA random priming technology amplifies fragmented or intact RNA from all sources including FFPE and RIP.
- Semi-degenerate library primer design for more complete transcriptome coverage and efficient priming.
- No need to fragment DNA before sequencing.
- Amplifies ds-cDNA in 8 hours or less.
- Compatible with all next generation sequencing platforms except Pacific Bioscience.
Otras notas
SEQR-500RXN is manufactured on-demand. Contact us for more information.
Información legal
SeqPlex is a trademark of Sigma-Aldrich Co. LLC
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Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
11 - Combustible Solids
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Protocolos
SEQR - SeqPlex RNA Amplification Kit Protocol
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