Saltar al contenido
Merck
Todas las fotos(2)

Key Documents

View All Documentation

WGA2

Sigma-Aldrich

GenomePlex® Complete Whole Genome Amplification (WGA) Kit

Optimized kit with enzyme for amplifying a variety of DNA including FFPE tissue

Sinónimos:

Whole genome amplification kit

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Todas las fotos(2)

About This Item

Código UNSPSC:
41121800
NACRES:
NA.55

Nivel de calidad

200

técnicas

whole genome amplification: suitable

Condiciones de envío

wet ice

temp. de almacenamiento

−20°C

Categorías relacionadas

Descripción general

GenomePlex® Complete Whole Genome Amplification Kit utilizes a proprietary technology based on random fragmentation of genomic DNA and conversion of the resulting small fragments to PCR-amplifiable library molecules flanked by universal priming sites. WGA is achieved by PCR amplification of the library molecules using universal oligonucleotide primers. It contains everything required for whole genome amplification including an optimized enzyme, WGA DNA Polymerase. It can be used in several applications and is suitable for use with purified genomic DNA from a variety of sources including blood cards, whole blood, buccal swabs, soil, plant, and serum. It allows the researcher to generate a representative, ~500-fold amplification of genomic DNA.

Aplicación

GenomePlex® Complete Whole Genome Amplification (WGA) Kit has been used in the amplification of DNA. This kit is also suitable for use with downstream applications including:
  • microarray analysis
  • SNP analysis
  • STR analysis
  • DNA archiving

Características y beneficios

  • Higher yield from minimal template: Amplification of nanogram amounts of genomic DNA to microgram yields (around 10 μg) in less than about three hoursrs
  • Nanograms of samples can be preserved at –20 °C for future use
  • Choose from a variety of DNA sources: whole blood, buccal swab, blood card, plant, soil, & formalin-fixed paraffin-embedded tissue (FFPE)
  • Whole-genome amplification (WGA) DNA polymerase increases the amplification accuracy
  • Whole-genome representation with no detectable allele bias
  • Compatible with many downstream applications such as TaqMan® assays, single nucleotide polymorphism (SNP) analysis, comparative genomic hybridization (CGH) analysis

Otras notas

The sequences of the universal primers provided in this kit are considered proprietary.

Información legal

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
GenomePlex is a registered trademark of Takara Bio USA, Inc.
TaqMan is a registered trademark of Roche Molecular Systems, Inc.

Los componentes del kit también están disponibles por separado

Referencia del producto
Descripción
SDS
  • W4502Water, Nuclease-Free Water, for Molecular BiologySDS

Pictogramas

Health hazard
GHS08

Palabra de señalización

Danger

Frases de peligro

H334

Consejos de prudencia

P261 - P284 - P501

Clasificaciones de peligro

Resp. Sens. 1

Código de clase de almacenamiento

12 - Non Combustible Liquids

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Levi Yant et al.
The Plant cell, 22(7), 2156-2170 (2010-08-03)
The Arabidopsis thaliana transcription factor APETALA2 (AP2) has numerous functions, including roles in seed development, stem cell maintenance, and specification of floral organ identity. To understand the relationship between these different roles, we mapped direct targets of AP2 on a
M Korabecna et al.
Neoplasma, 63(3), 402-410 (2016-03-02)
Tubulocystic renal cell carcinoma (TRCC) represents a rare tumor with incidence lower than 1 % of all renal carcinomas. This study was undertaken to contribute to characterization of molecular signatures associated with TRCC and to compare them with the features of papillary
Tao Chen et al.
Toxicology, 292(2-3), 63-70 (2011-11-15)
Furan, a widely used industrial compound, has been found in a number of heated food items. Furan is carcinogenic to rats and mice, but the mechanism behind its carcinogenic effect is still not well understood. In this study, we tested
Karolina Åberg et al.
European journal of human genetics : EJHG, 20(9), 953-955 (2012-03-02)
DNA from Epstein-Barr virus-transformed lymphocyte cell lines (LCLs) has proven useful for studies of genetic sequence polymorphisms. Whether LCL DNA is suitable for methylation studies is less clear. We conduct a genome-wide methylation investigation using an array set with 45
Zhong Zhao et al.
Nature, 465(7301), 1089-1092 (2010-06-26)
The classic phytohormones cytokinin and auxin play essential roles in the maintenance of stem-cell systems embedded in shoot and root meristems, and exhibit complex functional interactions. Here we show that the activity of both hormones directly converges on the promoters
Ver todos los artículos relacionados

Artículos

Oligonucleotide Array CGH Analysis of a Robust Whole Genome Amplification Method

In recent years, array-based Comparative Genomic Hybridization (aCGH) has been refined to determine chromosomal changes at progressively higher resolutions. This evolving technology is, however, somewhat hampered by the large DNA input requirement—a minimum of 150,000 copies of a human genome, or 0.5 μg, are generally needed per sample to process one CGH array.

Array CGH Analysis of Challenging Samples

In recent years, array-based Comparative Genomic Hybridization (aCGH) has been refined to determine chromosomal changes at progressively higher resolutions. This evolving technology is, however, hampered by the large DNA input requirement—a minimum of 150,000 copies of a human genome, or 0.5 μg, are generally needed per sample to rocess one CGH array.

Qualitative multiplex PCR assay for assessing DNA quality from FFPE tissues and other sources of damaged DNA

The assessment of DNA quality is a crucial first step in acquiring meaningful data from formalin-fixed paraffin-embedded (FFPE) tissues, and other sources of damaged DNA. Using intact genomic DNA is key for successful analysis of chromosomal aberrations (e.g. SNP analysis, LOH, aCGH, etc.).

Protocolos

Protocol for DNA extraction & WGA Amplification from Soil Samples

Genomic DNA from soil samples can be easily damaged by nucleases and contaminating debris resulting in low DNA yields. As a result, the researcher’s ability to perform downstream analysis may be compromised. After isolating DNA from the soil sample, the GenomePlex® Whole Genome Amplification Protocol is followed

Saliva DNA Extraction & WGA Amplification Protocol

This protocol provides a simple and convenient method to isolate, amplify and purify genomic DNA from saliva

Whole Genome Amplification from Serum or Plasma Protocol

Whole genome amplification (WGA) of plasma and serum DNA presents a unique challenge due to the small amount of nucleic acid in such samples.

Extraction & Amplification of Whole Blood Using WGA-Protocol

Whole blood is a common source of material used to perform genetic analysis. Many times genomic DNA isolated from whole blood samples is of low yield. This protocol is a simple method to isolate DNA from fresh or aged whole blood products. Once the DNA is isolated, it can be amplified using the GenomePlex® Whole Genome Amplification protocol.

Ver todo

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico