SAE0092
Mutanolisina from Streptomyces globisporus ATCC 21553
free of DNA contaminants, suitable for Microbiome research, lyophilized powder, ≥4000 units/mg protein (biuret)
Sinónimos:
Mutanolysin
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About This Item
Productos recomendados
origen biológico
Streptomyces sp. (Streptomyces globisporus ATCC 21553)
Formulario
lyophilized powder
actividad específica
≥4000 units/mg protein (biuret)
mol peso
23 kDa
~18.5 kDa
Características
DNA free
concentración
≥40% protein
técnicas
nucleic acid detection: suitable
idoneidad
suitable for cell lysis
aplicaciones
life science and biopharma
Condiciones de envío
wet ice
temp. de almacenamiento
−20°C
Categorías relacionadas
Aplicación
Proporciona una lisis celular suave para el aislamiento de biomoléculas y ARN fácilmente degradables de bacterias. Se ha utilizado en la formación de esferoplastos para el aislamiento del ADN.
Purified Mutanolysin SAE0092 undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.
Mutanolysin is a muralytic enzyme, suitable for usage in isolation of nucleic acids, from Gram-positive bacteria (e.g. Listeria, Lactobacillus, Lactococcus),(8) and also from other bacteria that are difficult to lyse with lysozyme only.
Mutanolysin is a muralytic enzyme, suitable for usage in isolation of nucleic acids, from Gram-positive bacteria (e.g. Listeria, Lactobacillus, Lactococcus),(8) and also from other bacteria that are difficult to lyse with lysozyme only.
Acciones bioquímicas o fisiológicas
La mutanolisina es una N-acetilmuramidasa. Al igual que la lisozima, es una enzima muralítica que rompe el enlace β-N-acetilmuramil-(1→4)-N-acetilglucosamina del polímero de la pared celular bacteriana peptidoglucano-polisacárido. Sus restos carboxi terminales están implicadas en el reconocimiento y la unión de polímeros de pared celular únicos. Mutanolisina lisa Listeria y otras bacterias grampositivas como Lactobacillus y Lactococcus.
La mutanolisina rompe el enlace β-N-acetilmuramil-(1→4)-N-acetilglucosamina del polímero de la pared celular bacteriana peptidoglucano-polisacárido. Lisa Listeria y otras bacterias grampositivas como Lactobacillus y Lactococcus.
Características y beneficios
Suitable for difficult-to-lyse samples.
Microbial DNA free, minimizing DNA contamination from reagents in workflow.
Suitable for the isolation of nucleic acids from Gram-positive bacteria, including Listeria, Lactobacillus, and Lactococcus.
Microbial DNA free, minimizing DNA contamination from reagents in workflow.
Suitable for the isolation of nucleic acids from Gram-positive bacteria, including Listeria, Lactobacillus, and Lactococcus.
Definición de unidad
One unit will produce a ΔA600 of 0.01 per minute at pH 6.0 at 37 °C in a 1 mL volume using a suspension of Streptococcus faecalis cell wall as substrate.
Forma física
Lyophilized powder containing Ficoll® and sodium succinate buffer salts
Información legal
Ficoll is a registered trademark of Cytiva
Palabra de señalización
Danger
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Resp. Sens. 1
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 2
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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Applied and environmental microbiology, 82(2), 596-607 (2015-11-15)
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G B Calandra et al.
Infection and immunity, 28(3), 1033-1037 (1980-06-01)
Group B streptococci, refractory to previously tested muralysins under physiological conditions, were successfully converted to protoplasts by use of a recently describede N-acetyl muramidase, mutanolysin, derived from a streptomycete. Purified enzyme was effective, but crude preparations, although degrading cell walls
K Yokogawa et al.
Antimicrobial agents and chemotherapy, 6(2), 156-165 (1974-08-01)
Mutanolysin partially purified from the culture filtrate of Streptomyces globisporus 1829 consists of two main lytic enzymes with an isoelectric point near pH 8.5 and 10, respectively, and proteolytic enzyme is associated with the latter lytic enzyme. Mutanolysin exhibited maximal
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