SAE0098

Lyticase from Arthrobacter luteus

free of DNA contaminants, suitable for Microbiome research, ≥2000 units/mg protein, lyophilized powder

• Sinónimos: (1,3)-β-D-Glucan endohydrolase, 1,3-β-Glucan glucohydrolase
• Número de CAS: 37340-57-1
• Número MDL: MFCD00131560
• Código UNSPSC: 12352202
• NACRES: NA.54

Propiedades

• origen biológico: bacterial (Arthrobacter luteus)
• formulario: lyophilized powder
• actividad específica: ≥2000 units/mg protein
• Características: DNA free
• técnicas: cell based assay: suitable
• idoneidad: suitable for cell lysis
• aplicaciones: diagnostic assay manufacturing
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C

Descripción

Descripción general

Yeast cells are difficult to disrupt because the cell walls may form capsules or resistant spores. DNA can be extracted from yeast by using lysing enzymes such as lyticase, chitinase, zymolase, and gluculase to induce partial spheroplast formation; spheroplasts are subsequently lysed to release DNA. Lyticase is preferred to digest cell walls of yeast and generate spheroplasts from fungi for transformation.

Reported to be useful for lysis of Ashbya, Candida, Debaryomyces, Eremothecium, Endomyces, Hansenula, Hanseniaspora, Kloeckera, Kluyveromyces, Lipomyces, Metschikowia, Pichia, Pullularia, Torulopsis, Saccharomyces, Saccharomycopsis, Saccharomycodes, and Schwanniomyces species.

Purified Lyticase SAE0098 undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.

Acciones bioquímicas o fisiológicas

Lyticase hydrolyzes poly-β(1→3)-glucose such as yeast cell wall glucan.

Definición de unidad

One unit will produce a ΔA₈₀₀ of 0.001 per min at pH 7.5 at 25 °C, using a suspension of yeast as substrate in a 3 mL reaction mixture.

Otras notas

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Información de seguridad

• Pictogramas: GHS08
• Palabra de señalización: Danger
• Frases de peligro: H334
• Consejos de prudencia: P261 - P284 - P304 + P340 - P342 + P311 - P501
• Clasificaciones de peligro: Resp. Sens. 1
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable