SAB4200844

Anti-Cholera toxin, B Subunit (CTxB) antibody, Mouse monoclonal

clone CTxB-24, purified from hybridoma cell culture

• Sinónimos: Anti-toxB
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• forma del anticuerpo: purified from hybridoma cell culture
• tipo de anticuerpo: primary antibodies
• clon: CTxB-24, monoclonal
• descripción: Research area: Microbiome
• reactividad de especies: Vibrio cholerae
• envase: antibody small pack of 25 μL
• concentración: ~1 mg/mL
• técnicas: immunoblotting: 1-2 μg/mL using His-tagged recombinant Cholera Toxin B subunit
• isotipo: IgG1
• Nº de acceso UniProt: P01556
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified

Descripción

Descripción general

Cholera toxin (CTx) also known as choleragen, is an enterotoxin produced by the Gram-negative bacterium Vibrio cholerae that naturally habitat in fresh or saltwater environments. Most of the V. cholerae species do not cause any disease in human, but few including serotypes O1 and O139 can cause cholera pandemic. These cases were described already in the 19th century.1 The V. cholerae virulence factors CtxA and CtxB are located at the CTX phage genome integrated within the bacterial chromosome. Since species virulence may change due to mutations and acquisition of virulence genes, the cholera pandemic has a major public health risk with potential for large numbers of cases and even deaths.1-3

Especificidad

Monoclonal Anti-Cholera Toxin-peroxidase antibody specifically recognizes Cholera Toxin and has no cross reactivity with Staphylococcal Enterotoxin A (SEA), Staphylococcal Enterotoxin B (SEB), Pseudomonas Exotoxin A or Staphylococcal Alpha-Toxin (α-Hemolysin).

Inmunógeno

recombinant Cholera toxin beta (C-terminal His-tag) protein.

Aplicación

The antibody may be used in various immunochemical techniques including Immunoblotting, Immunofluorescence and ELISA. Detection of the CTxB band by Immunoblotting is specifically inhibited by the immunogen.

Acciones bioquímicas o fisiológicas

CTx is composed of two subunits, the toxic CTxA (~27 kDa) and non-toxic CTxB (~12 kDa) assembled with the stoichiometry AB5.4 The B-subunit specifically binds to monosialogangliosides GM1 receptors, located in the membrane of intestinal epithelial cells.5 The A1 fragment of the A-subunit is translocated through the membrane of the host cell, where it catalyses the ADP-ribosylation of the Gsa regulatory component of the adenylate cyclase complex. The resulting increased level of cyclic AMP promotes a wide variety of actions, including the secretion of chloride ions in the case of intestinal epithelial cells.6-7 Antibodies specific for cholera toxin may be used in studies of structural and functional aspects of toxin-membrane interactions and for the detection of CTxB when used for example as an adjuvant when injected mucosally together with the desired antigen.8-10

Forma física

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

Almacenamiento y estabilidad

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable