C9903

Cholera Toxin B subunit

≥95% (SDS-PAGE), lyophilized powder, enterotoxin

• Sinónimos: CTB
• Número MDL: MFCD02356522
• Código UNSPSC: 12352200
• ID de la sustancia en PubChem: 329775212
• NACRES: NA.77

Propiedades

• product name: Cholera Toxin B subunit, ≥95% (SDS-PAGE), lyophilized powder
• Nivel de calidad: 300
• Análisis: ≥95% (SDS-PAGE)
• formulario: lyophilized powder
• mol peso: ~12 kDa
• composición: Protein, ~5% Lowry
• impurezas: ≤0.5% Cholera toxin A subunit (SDS-PAGE)
• solubilidad: H₂O: 10 mg/mL
• temp. de almacenamiento: 2-8°C
• cadena SMILES: CCOc1ccccc1C(=O)Nc2ccc(Cl)c(c2)C(F)(F)F
• InChI: 1S/C16H13ClF3NO2/c1-2-23-14-6-4-3-5-11(14)15(22)21-10-7-8-13(17)12(9-10)16(18,19)20/h3-9H,2H2,1H3,(H,21,22)
• Clave InChI: YDXZSNHARVUYNM-UHFFFAOYSA-N

Descripción

Descripción general

Cholera Toxin is a heterohexameric AB5 enterotoxin. Cholera Toxin B subunit is the the B subunit pentamer of cholera toxin produced by Vibrio cholera. It is generally involved in the cell trafficking and signalling machineries.

Aplicación

Cholera Toxin B subunit has been used:

• for macrophage stimulation and i.p. injection in a study to determine the endotoxin sensitivity of Caspase-4.
• in transganglionic and retrograde tract-tracing method combined with dual-immunofluorescence histochemistry of adult rat Vmes neuron cells.

Acciones bioquímicas o fisiológicas

The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport. Tissue culture cells treated with cholera toxin are not killed and tissues of animals do not become necrotic.

Características y beneficios

This compound is a featured product for Cyclic Nucleotide research. Click here to discover more featured Cyclic Nucleotide products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.

Envase

Package size based on protein content

Forma física

Lyophilized powder containing Tris buffer salts, sodium chloride, sodium azide, and sodium EDTA.

Reconstitución

When reconstituted with water to a final concentration of 1 mg of CTB per ml, the solution will contain 0.05 M Tris buffer, pH 7.5, 0.2 M NaCl, 3 mM NaN₃ and 1 mM sodium EDTA.

Nota de análisis

Activity measured by ELISA using ganglioside GM₁-coated multiwell plates, rabbit anti-Cholera toxin B subunit antibodies, and peroxidase-labeled goat anti-rabbit IgG as the secondary antibody. 50% saturation of binding was achieved with 0.05-1 μg of Cholera toxin B subunit per mL.

Información de seguridad

• Pictogramas: GHS07
• Palabra de señalización: Warning
• Frases de peligro: H312 + H332,H412
• Consejos de prudencia: P261 - P271 - P273 - P280 - P302 + P352 + P312 - P304 + P340 + P312
• Clasificaciones de peligro: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 3
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable