S8884

Safranin O

Dye content, ≥80%, certified by the Biological Stain Commission, powder

• Sinónimos: Basic Red 2, Cotton Red, Gossypimine, Safranin T, Safranin Y or A
• Fórmula empírica (notación de Hill): C₂₀H₁₉ClN₄
• Número de CAS: 477-73-6
• Peso molecular: 350.84
• Número de índice de color: 50240
• Beilstein: 3924099
• Número CE: 207-518-8
• Número MDL: MFCD00011759
• Código UNSPSC: 12171500
• ID de la sustancia en PubChem: 24899820
• NACRES: NA.47

Propiedades

• product name: Safranin O, certified by the Biological Stain Commission
• grado: certified by the Biological Stain Commission
• Nivel de calidad: 200
• formulario: powder
• composición: Dye content, ≥80%
• técnicas: microbe id | staining: suitable
• color: green to very dark green
• solubilidad: H₂O: 1 mg/mL
• aplicaciones: diagnostic assay manufacturing; hematology; histology
• temp. de almacenamiento: room temp
• cadena SMILES: [Cl-].Cc1cc2nc3cc(C)c(N)cc3[n+](-c4ccccc4)c2cc1N
• InChI: 1S/C20H18N4.ClH/c1-12-8-17-19(10-15(12)21)24(14-6-4-3-5-7-14)20-11-16(22)13(2)9-18(20)23-17;/h3-11H,1-2H3,(H3,21,22);1H
• Clave InChI: OARRHUQTFTUEOS-UHFFFAOYSA-N

Descripción

Descripción general

Safranin O is a metachromatic, cationic dye. It is used as a counterstain in Gram staining. The stain colors Gram-negative bacteria pink to red and has no effect on Gram-positive bacteria. It is also used to assess glycosaminoglycans (GAGs) in cartilage. The dye imparts an intense red to GAGs. It easily binds to polyanions.

Aplicación

Safranin O has been used in the staining of extraocular muscle mesenchymal stem cells (EOM-MSC) for the detection of cartilage, mucin and mast cell granules It has also been used as a mediator dye in cytochrome P450 spectral assay.

Safranin O has been used to stain matrix proteoglycans to study cartilaginous tissue structural integrity in mice. It has also been used to stain chondrogenically differentiated human bone marrow stromal cells.

Idoneidad

Certified as a stain for chromosomes in the Flemming triple stain; to stain gram negative bacteria in the Gram stain procedure; Safranine-fast green FCF stain for plant cells.

Información de seguridad

• Pictogramas: GHS05
• Palabra de señalización: Danger
• Frases de peligro: H318
• Consejos de prudencia: P280 - P305 + P351 + P338
• Clasificaciones de peligro: Eye Dam. 1
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Equipo de protección personal: dust mask type N95 (US), Eyeshields, Gloves