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84120

Sigma-Aldrich

Safranin O

for microscopy (Bact., Bot., Hist.), indicator (pH 0.3-1.0)

Sinónimos:

Basic Red 2, Cotton Red, Gossypimine, Safranin T, Safranin Y or A

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About This Item

Fórmula empírica (notación de Hill):
C20H19ClN4
Número de CAS:
477-73-6
Peso molecular:
350.84
Número de índice de color:
50240
Beilstein:
3924099
Número CE:
207-518-8
Número MDL:
MFCD00011759
Código UNSPSC:
12171500
ID de la sustancia en PubChem:
329769290
NACRES:
NA.47

grado

for microscopy (Bact., Bot., Hist.)
indicator (pH 0.3-1.0)

formulario

powder or crystals

técnicas

titration: suitable

pH

10 (20 °C, 10 g/L)

solubilidad

methanol: 0.01 g/10 mL, red

εmax

≥1000 at 525-535 nm in 50% ethanol

aplicaciones

diagnostic assay manufacturing
hematology
histology
microbiology

temp. de almacenamiento

room temp

cadena SMILES

[Cl-].Cc1cc2nc3cc(C)c(N)cc3[n+](-c4ccccc4)c2cc1N

InChI

1S/C20H18N4.ClH/c1-12-8-17-19(10-15(12)21)24(14-6-4-3-5-7-14)20-11-16(22)13(2)9-18(20)23-17;/h3-11H,1-2H3,(H3,21,22);1H

Clave InChI

OARRHUQTFTUEOS-UHFFFAOYSA-N

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Descripción general

Safranin O, also known as Basic Red 2, is a basic, cationic, and metachromatic azine dye used as a biological stain. The characteristic azine chromophore contains the phenazine, a planar, tricyclic dibenzopyrazine, which consists of two non-planar benzene rings linked by two nitrogen atoms. A delocalized positive charge, an alternation of aromatic and quinonoid structures, and diazotizable amine groups constitute the chromophoric system. c-Methylation makes this dye of moderate overall size. The trimethyl cationic form of safranine is lipophilic, whereas the dimethyl forms are weakly hydrophilic. The dye is commercially available as a chloride.

Aplicación

  • Safranin O is widely employed as a microbiological stain, especially as a counterstain in Gram staining to distinguish Gram-positive from Gram-negative micro-organisms, and for staining bacterial spores.
  • It is used to demonstrate glycosaminoglycans in human and animal histological specimens and nuclei.
  • It is a component of Benda′s polychrome stain.
  • It is a component of various polychromes and lignin stains for botanical staining applications.
  • It has also been applied for the demonstration of mast cells in cytospin preparations and staining surgical frozen sections.

Pictogramas

Corrosion
GHS05

Palabra de señalización

Danger

Frases de peligro

H318

Consejos de prudencia

P280 - P305 + P351 + P338

Clasificaciones de peligro

Eye Dam. 1

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Equipo de protección personal

dust mask type N95 (US), Eyeshields, Gloves

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