89640

Toluidine Blue

8.74% (ZN (THEORY)), for microscopy (Hist., Vit.)

• Sinónimos: Tolonium chloride; Basic Blue 17
• Fórmula empírica (notación de Hill): C₁₅H₁₆ClN₃S · 0.5ZnCl₂
• Número de CAS: 6586-04-5
• Peso molecular: 373.97
• Número de índice de color: 52040
• Beilstein: 4068052
• Número MDL: MFCD03548293
• Código UNSPSC: 12171500
• ID de la sustancia en PubChem: 329769757
• NACRES: MA.02

Propiedades

• grado: for microscopy (Hist., Vit.)
• Nivel de calidad: 200
• Análisis: 8.74% (ZN (THEORY))
• formulario: powder or crystals
• color: green to very dark green
• solubilidad: methanol: 0.01 g/10 mL, blue to very deep blue
• εmax: ≥800 at 621-636 nm in water
• aplicaciones: diagnostic assay manufacturing; hematology; histology
• temp. de almacenamiento: room temp
• cadena SMILES: [Cl-].[Cl-].Cl[Zn]Cl.Cc1cc2N=C3C=CC(\C=C3Sc2cc1N)=[N+](\C)C.Cc4cc5N=C6C=CC(\C=C6Sc5cc4N)=[N+](\C)C
• InChI: 1S/2C15H15N3S.4ClH.Zn/c2*1-9-6-13-15(8-11(9)16)19-14-7-10(18(2)3)4-5-12(14)17-13;;;;;/h2*4-8,16H,1-3H3;4*1H;/q;;;;;;+2/p-2
• Clave InChI: DAQXTSXITPUGRG-UHFFFAOYSA-L

Descripción

Descripción general

Toluidine blue (C.l. 52040) is a diaminothiazine dye.It is also known as TBO, tolonium chloride, and toluidine blue O.

Aplicación

Toluidine Blue is suitable for use in the following applications:

1. to demonstrate amyloid; DNA in plant material, mast cells, Nissl substance, nuclei, and sulfatides
2. in metachromatic staining of sulfated mucins and related materials
3. as staining agent to identify the development and accumulation of the cartilage matrix, proteoglycans for isolation and culture of mesenchymal stem cells from mouse compact bone.
4. staining of mucins in water-miscible resins and of nucleic acids in epoxy resins(1)

Acciones bioquímicas o fisiológicas

Toluidine Blue is a basic thiazine metachromatic dye, which is used for selectively staining acidic tissue components such as carboxylates, sulfates, and phosphate radicals.It is found to have an affinity for nucleic acids and therefore binds to the nuclear material of tissues with a high DNA and RNA content.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)