T9284

Triton^™ X-100

BioXtra

• Sinónimos: t-Octilfenoxipolietoxietanol, Éter polietilenglicol terc-octilfenil
• Fórmula lineal: t-Oct-C₆H₄-(OCH₂CH₂)_xOH, x= 9-10
• Número de CAS: 9036-19-5
• Beilstein: 2315025
• Número MDL: MFCD00128254
• Código UNSPSC: 12161900
• ID de la sustancia en PubChem: 329826903
• NACRES: NA.21

Propiedades

• origen biológico: synthetic
• Nivel de calidad: 200
• descripción: non-ionic
• Línea del producto: BioXtra
• formulario: liquid
• mol peso: micellar avg mol wt 80,000; average mol wt 625
• número de agregación: 100-155
• técnicas: permeability assay: suitable
• impurezas: ≤0.05% Phosphorus (P)
• residuo de ign.: ≤0.4%
• CMC: 0.2-0.9 mM (20-25°C)
• temperatura de transición: cloud point 65 °C; pour point ~7 °C
• solubilidad: water: soluble
• densidad: 1.065 g/cm³ at 20 °C (68 °F)
• trazas de anión: chloride (Cl^-): ≤0.05%; sulfate (SO₄^2-): ≤0.05%
• trazas de catión: Al: ≤0.0005%; Ca: ≤0.0005%; Cu: ≤0.0005%; Fe: ≤0.0005%; K: ≤0.005%; Mg: ≤0.0005%; NH₄⁺: ≤0.05%; Na: ≤0.1%; Pb: ≤0.001%; Zn: ≤0.0005%
• HLB: 13.5
• cadena SMILES: CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1
• InChI: 1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3
• Clave InChI: HNLXNOZHXNSSPN-UHFFFAOYSA-N

Descripción

Descripción general

Triton^™ X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.

Aplicación

Triton^™ X-100 has been used:

• Along with Span^® 80 and Tween^® 80 in mineral oil for the preparation of oil-phase of microemulsions for PCR technique
• As a component of NX buffer which is used to pool and break down the microemulsions after PCR cycling
• In the blocking solution during immunocytochemistry procedure
• During deglycosylation procedure
• For membrane solubilization
• For cell permeabilization
• In decellularization of porcine heart valve tissue

Acciones bioquímicas o fisiológicas

Tensioactivo no iónico ampliamente utilizado para la recuperación de los componentes de la membrana bajo condiciones desnaturalizantes suaves.

Características y beneficios

• BioXtra Grade product for your Cell Biology and Biochemical research
• Non-ionic nondenaturing detergent
• Superior wetting agent and emulsifier
• Highly versatile surfactant

Otras notas

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

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Información legal

Span is a registered trademark of Croda International PLC

TWEEN is a registered trademark of Croda International PLC

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Información de seguridad

• Pictogramas: GHS05,GHS07,GHS09
• Palabra de señalización: Danger
• Frases de peligro: H302,H315,H318,H410
• Consejos de prudencia: P264 - P273 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Clasificaciones de peligro: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2
• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): 483.8 °F - closed cup
• Punto de inflamabilidad (°C): 251 °C - closed cup
• Equipo de protección personal: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter