S1816
SYBR® Green JumpStart™ Taq ReadyMix™ for Quantitative PCR, Capillary Formulation
SYBR® Green qPCR reagent for Roche LightCycler® capillary systems
About This Item
Productos recomendados
formulario
liquid
uso
sufficient for 100 reactions
sufficient for 400 reactions
Características
dNTPs included
hotstart
concentración
1 units/reaction (20 μL reaction volume)
técnicas
qPCR: suitable
color
colorless
entrada
purified DNA
compatibilidad
for use with Roche LightCycler 480
método de detección
SYBR® Green
Condiciones de envío
wet ice
temp. de almacenamiento
−20°C
Descripción general
SYBR Green Taq ReadyMix is recommended for single product real-time amplification experiments and may also be used for evaluation of primer sequences prior to manufacture of fluorescent-labeled probes. Fluorescent labeled probes are not recommended for use with SYBR Green I dye.
Aplicación
Características y beneficios
- Convenient 2× concentrate ReadyMix specifically designed for use with capillary instruments such as the Roche LightCycler® and is ideal for high throughput applications
- Increased specificity & target yield - JumpStart™ Taq polymerase prevents non-specific product resulting in more accurate CT values and improved quantitation
- This master mix allows consistency from one reaction to the next
- Designed to reduce the preparation time and minimize contamination from multiple pipetting steps
- The double-strand DNA-specific SYBR® Green I fluorescent dye is inexpensive, easy to use, and sensitive and is ideal for quantifying any DNA sequence
Envase
100RXN is packaged as 1 X 1 mL
400RXN is packaged as 1 X 4 mL
Principio
The JumpStart Taq antibody inactivates the DNA polymerase at room temperature. When the temperature is raised above 70 °C in the first denaturation step of the cycling process, the complex dissociates and the polymerase becomes fully active. JumpStart Taq DNA polymerase prevents non-specific amplification resulting in more accurate CT values.
To prepare a reaction, 10 μL of ReadyMix is added to primers, template and water for a final reaction volume of 20 μL.
Definición de unidad
Información legal
Producto relacionado
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Artículos
Quantitative PCR (qPCR) provides information about gene expression, gene amplification or loss, and small alterations. qPCR is often used to investigate tumor biology and to discover the genetic and epigenetic causes of cancer
The polymerase chain reaction is one of the most widely used techniques in molecular biology. The PCR process consists of three main steps, Denaturation, Annealing & Extension
Protocolos
A protocol that can be used as a basic template for qPCR incorporating SYBR Green I DNA binding dye that is amenable to modification and applicable for use as validation for a set of primers.
Our SYBR Green qPCR Protocol is a method designed to detect accurate quantification of gene expression and RT-PCR reactions
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