QR0100
SYBR® Green Quantitative RT-qPCR Kit
One step SYBR® Green RT-qPCR with MMLV & hot-start Taq DNA Polymerase
Sinónimos:
one step rt qPCR, sybr green qPCR
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About This Item
Productos recomendados
Nivel de calidad
uso
sufficient for 250 reactions
Características
dNTPs included
hotstart
técnicas
RT-qPCR: suitable
color
colorless
entrada
purified RNA
compatibilidad
ABI 5700
ABI 7000
ABI 7300
ABI 7500 Fast
ABI 7500
ABI 7700
ABI 7900 HT
ABI 7900 HT Fast
ABI 7900
ABI StepOne
ABI StepOnePlus
ABI ViiA 7
Bio-Rad CFX384
Bio-Rad CFX96
Bio-Rad MJ Chromo4
Bio-Rad MJ Opticon 2
Bio-Rad MJ Opticon
Bio-Rad MiniOpticon
Bio-Rad MyiQ
Bio-Rad iCycler iQ
Bio-Rad iQ5
Cepheid SmartCycler
Eppendorf® Mastercycler ep realplex2 s
Eppendorf® Mastercycler ep realplex
Illumina Eco qPCR
Qiagen Corbett Rotor-Gene 3000
Qiagen Corbett Rotor-Gene 6000
Qiagen Corbett Rotor-Gene Q
Roche LightCycler 480
Strategene Mx3000P
Strategene Mx3005P
Strategene Mx4000
método de detección
SYBR® Green
Condiciones de envío
wet ice
temp. de almacenamiento
−20°C
Categorías relacionadas
Descripción general
Aplicación
- to detect specific genetic clusters of genogroup I and II noroviruses
- for chikungunya viral (CHIKV) RNA quantification
- to detect mRNA expression levels
- for amplification of total RNA extracted from human umbilical vein endothelial cells (HUVECs) and prostate cancer cells
Características y beneficios
- The master mix allows consistency and reproducibility from one reaction to the next
- Reduced preparation time and the risk of contamination from multiple pipetting steps
- Reduced set-up time as compared to manual or wax Hot Start methods
- JumpStart™Taq Polymerase reduces primer-dimer and non-specific product formation
- SYBR® Green I dye is inexpensive, easy to use, and highly sensitive
- Broad instrument compatibility
- Includes a separate ROX reference dye vial for reaction normalization
Envase
Información legal
Solo componentes del kit
- SYBR® Green Taq ReadyMix™ for Quantitative RT-PCR 2 x 25
Palabra de señalización
Danger
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1C - Skin Sens. 1
Código de clase de almacenamiento
8A - Combustible corrosive hazardous materials
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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Artículos
Sigma’s RT-qPCR products combine the effective of Reverse Transcriptase with hot-start taq-directed antibody in convenient ReadyMixes for probe-based or SYBR® Green based applications.
Quantitative PCR (qPCR) provides information about gene expression, gene amplification or loss, and small alterations. qPCR is often used to investigate tumor biology and to discover the genetic and epigenetic causes of cancer
One approach to the analysis of gene expression is to measure the concentration of mRNA of a gene. There are several challenges to such analyses, such as the differences in half life between different transcripts, the temporal patterns of transcription and the lack of correlation between mRNA and protein.
A PCR master mix is a batch of PCR or RT-PCR reagents that can be divided among many PCR reaction tubes. It usually includes DNA polymerase, dNTPs, MgCl2 and buffer. Make your own master mix or choose a commercial one.
Protocolos
Primer Concentration Optimization Protocol is an approach to create a matrix of reactions. This is used to test a range of concentrations for each primer against different concentrations of the partner primer.
Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.
Coronavirus qPCR Primer and probe sets for the detection of SARS-CoV-2 (Corona Virus), with additional real-time RT-PCR, RT-qPCR and supporting reagents available.
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
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