PP0500
Protease Profiler™ Kit
Proteases for Mass Spectrometry and Proteomics
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About This Item
Código UNSPSC:
12352200
NACRES:
NA.56
Productos recomendados
calidad
Proteases for Mass Spectrometry and Proteomics
temp. de almacenamiento
−20°C
Descripción general
For detailed characterization of proteins of interest, increase your protease selection with the Protease Profiler. The Protease Profiler provides four proven alternative proteases in addition to Proteomics Grade Trypsin, an enzyme solublization reagent, and an enzyme reaction buffer. Each component has been stringently purified and qualified for MALDI-MS to ensure optimal performance. Characterize with greater flexibility, perform double enzymatic digestions, and explore alternative cleavage sites with the Protease Profiler.
- Double Enzymatic Digestion - An initial digestion may result in peptide fragments greater than 5 kDa, often above the optimum detection range for most MS systems/instruments. A second digestion using a protease with different specificity results in cleavage of the large peptides, generating smaller peptides suitable for MS.
- Explore Alternative Proteases - Trypsin may be incompatible with some protein samples. Find the protease best suited to your protein of interest and improve the quality of your data.
- Perform Either Solution or Gel-based Digests - Optimized protocols and reagents for both digest conditions are provided, enabling flexibility in your analysis.
Aplicación
The Protease Profiler™ Kit can be used for different enzymatic digestions on the proteins of interest.
Almacenamiento y estabilidad
The entire Protease Profiler™ Kit retains activity for at least 1 year when stored at –20 °C. Alternatively, some of the components (Trypsin, Lys-C, Asp-N, Trypsin Solubilization Reagent, and Enzyme Reaction Buffer) may be stored at 2–8 °C. The storage temperature indicated on the labels of these products is 2–8 °C. Storage at –20 °C will not compromise the performance of these products.
Información legal
Protease Profiler is a trademark of Sigma-Aldrich Co. LLC
Solo componentes del kit
Referencia del producto
Descripción
- Enzyme Solublization Reagent 1 mL
- Enzyme Reaction Buffer 25 mL
Los componentes del kit también están disponibles por separado
Referencia del producto
Descripción
SDS
- T6567Trypsin from porcine pancreas, Proteomics Grade, BioReagent, Dimethylated 20 μgSDS
- P3303Endoproteinase Asp-N from Pseudomonas fragi mutant strain, suitable for protein sequencing, lyophilized powder 2 μgSDS
- P6181Endoproteinase Glu-C from Staphylococcus aureus V8, suitable for protein sequencing, lyophilized powder 25 μgSDS
- P6056Endoproteinase Arg-C from mouse submaxillary gland, suitable for protein sequencing, lyophilized powder 5 μgSDS
Palabra de señalización
Danger
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Acute Tox. 4 Oral - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3
Órganos de actuación
Respiratory system
Código de clase de almacenamiento
10 - Combustible liquids
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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