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Merck

T7575

Sigma-Aldrich

Trypsin Singles, Proteomics Grade

Sinónimos:

Proteomics grade, Trypsin, TPCK treated

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About This Item

Código UNSPSC:
12352204
NACRES:
NA.56

grado

Proteomics Grade

Nivel de calidad

formulario

ready-to-use solution

actividad específica

≥10,000 BAEE units/mg protein

mol peso

average mol wt 23.29 kDa

pH óptimo

~8.0

temp. de almacenamiento

2-8°C

Descripción general

Experience all of the advantages of Proteomics Grade Trypsin in a convenient, single-use 1 μg package.

Trypsin Singles, Proteomics Grade exhibit excellent proteolytic efficiency, generating more tryptic peptides leading to greater sequence coverage of your peptide of interest. Mass spectra are significantly simplified due to the reduced number of interfering autolytic peaks and their ambiguous adducts. Proteomics Grade Trypsin has been extensively purified from porcine pancreas to enable accurate and precise cleavage on the carboxylic side of Arg and Lys residues. The enzyme has been exhaustively processed by reductive methylation to minimize autolysis and chymotryptic activity has been quenched by TPCK treatment. Further purification steps including affinity chromatography and drying from dilute acid produce a highly purified, high specific activity trypsin purposely suited for the demanding criteria of proteomics research, and designed to function in either solution or gel-based digests.

Aplicación

Trypsin Singles, Proteomics Grade have been used for protein digestion to analyse generated peptides via MALDI-TOF MS (matrix assisted laser desorption ionization-time of flight mass spectrometry). It has been used for the digestion of proteins to analyse generated peptides via LC-MS (liquid chromatography–mass spectrometry)/MS.

Características y beneficios

  • Convenient, single-use 1 μg size
  • Eliminates repetitive pipetting
  • Reductively methylated to minimize autolytic activity
  • TPCK treated to quench chymotryptic activity
  • Extensively purified

Los componentes del kit también están disponibles por separado

Referencia del producto
Descripción
SDS

  • Trypsin Solubilization Reagent

Producto relacionado

Referencia del producto
Descripción
Precios

Palabra de señalización

Danger

Clasificaciones de peligro

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Órganos de actuación

Respiratory system

Código de clase de almacenamiento

3 - Flammable liquids


Certificados de análisis (COA)

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