P9290
Proteinase K–Agarose from Tritirachium album
lyophilized powder
Sinónimos:
Proteinase K-Agarose
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About This Item
Productos recomendados
origen biológico
microbial (T.album
T. ALBUM)
Nivel de calidad
Formulario
lyophilized powder
Extensión del etiquetado
30-140 units per g solid
técnicas
DNA extraction: suitable
RNA extraction: suitable
protein extraction: suitable
hinchazón
1 g swells to ~5 mL
temp. de almacenamiento
−20°C
Aplicación
Proteinase K-agarose is used in protein and nucleic acid isolation, modifying enzymes and immobilized enzymes. Proteinase K-agarose has been used to study Klebsiella pneumonia as well as Echinococcus multilocularis infection in domesticated dogs.
Acciones bioquímicas o fisiológicas
The application of proteinase K has been extended by immobilizing it on an insoluble matrix such as agarose. The agarose is quite inert in most systems and allows high activities per unit weight. This reactive resin allows the enzyme reaction to be quickly catalyzed by momentary contact with the substrate in a suitable medium. Reactions can be carried out in a batch slurry or even through a small column. In either case, the insoluble enzyme does not contaminate the reaction mix, which can be readily filtered out of the batch slurry or remain behind in the column.
Características y beneficios
• Recylable Proteinase K on matrix support.
• Spin column or batch processing possible.
• Spin column or batch processing possible.
Definición de unidad
One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).
Forma física
Lyophilized powder stabilized with lactose
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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Experimental parasitology, 128(1), 50-56 (2011-02-09)
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