N3129

4-Nitrophenyl phosphate bis(cyclohexylammonium) salt

phosphatase substrate, chromogenic, ≥97% anhydrous basis, powder

• Sinónimos: p-Nitrophenyl phosphate bis(cyclohexylammonium) salt, PNPP
• Fórmula lineal: O₂NC₆H₄OP(O)(O^-)₂ · 2C₆H₁₁NH₃⁺
• Número de CAS: 52483-84-8
• Peso molecular: 417.44
• Beilstein: 6553276
• Número CE: 257-949-0
• Número MDL: MFCD00013173
• Código UNSPSC: 12352204
• ID de la sustancia en PubChem: 24897623
• NACRES: NA.83

Propiedades

• Nombre del producto: 4-Nitrophenyl phosphate bis(cyclohexylammonium) salt, phosphatase substrate
• Nivel de calidad: 300
• Ensayo: ≥97% anhydrous basis
• Formulario: powder
• solubilidad: water: 10 mg/mL, clear, colorless to faintly yellow
• temp. de almacenamiento: −20°C
• cadena SMILES: NC1CCCCC1.NC2CCCCC2.OP(O)(=O)Oc3ccc(cc3)[N+]([O-])=O
• InChI: 1S/C6H6NO6P.2C6H13N/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*7-6-4-2-1-3-5-6/h1-4H,(H2,10,11,12);2*6H,1-5,7H2
• Clave InChI: KXNNUYCXODNFBD-UHFFFAOYSA-N

Descripción

Aplicación

4-Nitrophenyl phosphate bis(cyclohexylammonium) salt has been used as a component in the colorimetric reagent for abscisic acid (ABA) assay.

Acciones bioquímicas o fisiológicas

p-Nitrophenyl phosphate (pNPP) is the preferred substrate for use with alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA) procedures. Due to its high sensitivity, pNPP is the considered substrate in alkaline phosphatase systems. ELISA applications based on pNPP can be read in timed assays or delayed readings by stopping with alkaline solutions. This substrate yields a yellow-colored soluble end product that can be measured spectrophotometrically at 405 nm.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)