M1636

Myosin, Calcium activated from rabbit muscle

buffered aqueous glycerol solution, 0.5-1.5 units/mg protein (biuret)

• Sinónimos: Myosin adenosinetriphosphatase
• Número MDL: MFCD00131645
• Código UNSPSC: 12352202
• NACRES: NA.32

Propiedades

• origen biológico: rabbit muscle
• Nivel de calidad: 200
• formulario: buffered aqueous glycerol solution
• actividad específica: 0.5-1.5 units/mg protein (biuret)
• mol peso: heavy chain ~200 kDa (each); light chain 15-20 kDa (each); ~480 kDa
• técnicas: ELISA: suitable
• Condiciones de envío: wet ice
• temp. de almacenamiento: −20°C

Descripción

Acciones bioquímicas o fisiológicas

Myosin interacts with actin in muscle and non-muscle cells. Myosin molecules consist of two major regions: tails (rods) and heads. They aggregate into filaments through the tail region and interact with actin and with ATP through the head region. Myosin molecules spontaneously assemble into filaments in solutions of physiologic ionic strength and pH. Thick filament consists mainly of myosin molecules. Myosin is activated by the enzyme ATPase. This activation is the immediate source of the free energy that drives muscle contraction. It binds to the polymerized form of actin, the major constituent of the thin filament. Multiple forms of myosin heavy chains exist for each muscle type-skeletal, cardiac, smooth and non-muscle isomyosin forms exist in different types of skeletal muscle, depending on the physiological function of the muscle. These are designated as type I (slow-twitch) and type II (fast-twitch). Myosin is one of three classes of cytoskeletal motor proteins that have been identified. The others are kinesins and dyneins. These three types of proteins are thought to be responsible for the many movements that occur in cells. Myosin has been shown to be involved in neurosensory function, vesicle trafficking, determinant partitioning, and cortical function.

Myosin interacts with actin in muscle and non-muscle cells. Myosin is one of three classes of cytoskeletal motor proteins that have been identified. The others are kinesins and dyneins. These three types of proteins are thought to be responsible for the many movements that occur in cells. Myosin has been shown to be involved in neurosensory function, vesicle trafficking, determinant partitioning, and cortical function.

Definición de unidad

One unit will liberate 1.0 μmole of inorganic phosphorus from ATP per min at pH 9.0 at 25 °C in the presence of calcium.

Forma física

Solution in 50% glycerol containing 0.6 M potassium chloride and 10 mM potassium phosphate buffer, pH 6.8. Note: Solution may be hazy.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)