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L7533

Sigma-Aldrich

LB Broth with agar (Lennox)

EZMix powder microbial growth medium

Sinónimos:

LB broth

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About This Item

Código UNSPSC:
41106200
NACRES:
NA.85

grado

for molecular biology

Nivel de calidad

200

esterilidad

non-sterile

formulario

powder

composición

Agar, 15 g/L
NaCl, 5 g/L
Tryptone, 10 g/L
Yeast Extract, 5 g/L

técnicas

microbiological culture: suitable

aplicaciones

agriculture
food and beverages
microbiology

temp. de almacenamiento

room temp

idoneidad

nonselective for Escherichia coli
nonselective for coliforms

Descripción general

Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation. The addition of agar provides a solid medium for microbial growth.

Aplicación

Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin®.

Características y beneficios

Lennox EZMix powder with agar provides:
  • A budget-friendly alternative to pre-poured plates
  • Granulated, dust-free format for safer handling and faster mixing
  • Convenient small package to eliminate weighing
  • Easy scale-up using larger package sizes
  • Standard formulation

Nota de preparación

1. Suspend 35.6 g in 1 L of distilled water.
2. Heat to boiling while stirring to dissolve all ingredients completely.
3. Autoclave for 15 minutes at 121°C.
To prepare Lennox L Agar: Add 1 g glucose and proceed with preparation instructions as above.
To prepare the medium of Enquist and Sternberg: Aseptically add 10 ml of sterile 1 M magnesium sulfate after autoclaving.

Reconstitución

Add 35.6g powder in 1L water. Heat to boiling while stirring to dissolve powder. Autoclave for 15 minutes at 121C to sterilize. Allow to cool slightly before making additions, such as antibiotics (if desired). Pour into petri dishes and allow to solidify.

Información legal

Zeocin is a registered trademark of Cayla Sarl

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Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Sambrook, J., et al
Molecular Cloning: A Laboratory Manual, 2, 1-1 (1989)
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