I6758

IPTG

≥99% (TLC), ≤0.1% Dioxane

• Sinónimos: Isopropyl β-D-1-thiogalactopyranoside, IPTG, Isopropyl β-D-thiogalactoside
• Fórmula empírica (notación de Hill): C₉H₁₈O₅S
• Número de CAS: 367-93-1
• Peso molecular: 238.30
• Beilstein: 4631
• Número CE: 206-703-0
• Número MDL: MFCD00063273
• Código UNSPSC: 12352201
• ID de la sustancia en PubChem: 24896093
• NACRES: NA.31

Propiedades

• origen biológico: synthetic
• Nivel de calidad: 300
• descripción: non-ionic
• Análisis: ≥99% (TLC)
• formulario: powder
• técnicas: thin layer chromatography (TLC): suitable
• impurezas: ≤0.1% Dioxane
• color: white to off-white
• intervalo de pH útil: 5-7 (50 g/L)
• mp: 105 °C ((221 °F))
• solubilidad: water: 0.05 g/L, clear
• aplicaciones: cell analysis; life science and biopharma
• temp. de almacenamiento: 2-8°C
• cadena SMILES: CC(C)S[C@@H]1OC(CO)[C@H](O)C(O)[C@H]1O
• InChI: 1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1
• Clave InChI: BPHPUYQFMNQIOC-NXRLNHOXSA-N

Descripción

Descripción general

Isopropyl β-D-Thiogalactopyranoside (IPTG) is a molecular biology reagent used to induce gene expression in bacterial systems. It mimics lactose, binding to the lac repressor protein, leading to gene activation. IPTG is crucial for studying gene regulation, protein expression, and recombinant protein production, providing valuable insights into cellular and molecular biology research.

Aplicación

IPTG has been used:

• in the induction of protein expression in Lactobacillus reuteri GroEL and mutants
• in the induction of protein expression in Escherichia coli

IPTG is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ.

Acciones bioquímicas o fisiológicas

IPTG is a non-metabolizable analog of allolactose. It acts as an inducer that regulates the transcriptional activity of the lac promoter, a promoter that is used for the expression of the protein in E.coli.

Otras notas

Non-metabolizable galactose analog.

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To gain a comprehensive understanding of our extensive range of Monosaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Reconstitución

A stock solution (0.1 M) is prepared by dissolving IPTG in water with subsequent sterile filtration of the solution. The final concentration of IPTG in indicator plates should be 0.2 mM.

Información de seguridad

• Pictogramas: GHS08
• Palabra de señalización: Danger
• Frases de peligro: H350
• Consejos de prudencia: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Clasificaciones de peligro: Carc. 1B
• Código de clase de almacenamiento: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)