IPTG-RO
Roche
Isopropyl-β-D-thiogalactoside
mol wt 238.3, pkg of 1 g (10724815001), pkg of 5 g (11411446001)
Sinónimos:
Isopropyl β-D-1-thiogalactopyranoside, iptg, IPTG, Isopropyl β-D-thiogalactoside
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About This Item
Fórmula empírica (notación de Hill):
C9H18O5S
Número de CAS:
Peso molecular:
238.30
Beilstein:
4631
Número MDL:
Código UNSPSC:
12352200
ID de la sustancia en PubChem:
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Formulario
powder
mol peso
238.3
envase
pkg of 1 g (10724815001)
pkg of 5 g (11411446001)
fabricante / nombre comercial
Roche
Condiciones de envío
wet ice
temp. de almacenamiento
2-8°C
cadena SMILES
CC(C)S[C@@H]1OC(CO)[C@H](O)C(O)[C@H]1O
InChI
1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1
Clave InChI
BPHPUYQFMNQIOC-NXRLNHOXSA-N
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Descripción general
Isopropyl-β-D-thiogalactoside (IPTG) is a chemical analog of galactose that cannot be cleaved by β-galactosidase. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ.
Aplicación
IPTG is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ.
Isopropyl-β-D-thiogalactoside (IPTG) is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. It has been used for the expression of recombinant genes in E. coli.
Otras notas
Non-metabolizable galactose analog.
For life science research only. Not for use in diagnostic procedures.
The amount of labeled DNA depends on:
- amount of template DNA
- purity of template DNA
- conformation of template DNA
- average size of labeled fragment
Especificaciones
Assay Time: 50minutes
Specific Activity: The described standard assay will results in a specific activity of 1.8 x 109 dpm/μg, corresponding to 65% incorporation with different substrate DNAs in 30minutes. When varying the ratio of template DNA to labeled dNTP, similar incorporation rates, but different levels of specific activity of the labeled probe are obtained.
Specific Activity: The described standard assay will results in a specific activity of 1.8 x 109 dpm/μg, corresponding to 65% incorporation with different substrate DNAs in 30minutes. When varying the ratio of template DNA to labeled dNTP, similar incorporation rates, but different levels of specific activity of the labeled probe are obtained.
Nota de preparación
Sample Material
10 ng to 3μg template DNA, linearized and at least 100 to 200bp long
10 ng to 3μg template DNA, linearized and at least 100 to 200bp long
Working concentration: Final concentration should be 0.2 mM.
Storage conditions (working solution): Stock solutions of the product in water (e.g., 1 M IPTG) stored at -15 to -25 °C, are stable up to six months.
Storage conditions (working solution): Stock solutions of the product in water (e.g., 1 M IPTG) stored at -15 to -25 °C, are stable up to six months.
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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The role of the Escherichia coli lacY gene product (the lactose permease) in the induction of isopropyl-beta-D-thiogalactopyranoside (IPTG) inducible promoters was studied in E. coli and P. fluorescens. This was done by comparing strains containing a lacIPOZYA chromosomal insert with
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