L3397

LB Broth (Luria low salt)

Powder microbial growth medium

• Sinónimos: Luria Broth (Miller’s Modification), Luria-Bertani medium (LB)
• Código UNSPSC: 41106200
• NACRES: NA.85

Propiedades

• grado: for molecular biology
• Nivel de calidad: 200
• esterilidad: non-sterile
• formulario: powder
• composición: NaCl, 0.5 g/L ; Tryptone, 10 g/L ; Yeast Extract, 5 g/L
• técnicas: microbiological culture: suitable
• pH: 6.8-7.2(1.55% solution)
• idoneidad: suitable for molecular biology; nonselective for Escherichia coli; nonselective for coliforms
• aplicaciones: food and beverages; microbiology
• temp. de almacenamiento: room temp

Descripción

Descripción general

Luria Broth is a bacterial culture medium used for the cultivation of members of the Enterobacteriaceae especially E.coli, as well as for coliphage plaque assays. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a very low-salt formulation.

Aplicación

LB Broth (Luria low salt) has been used as a liquid broth medium in the broth microdilution technique.

Características y beneficios

Luria′s low salt LB powder provides:

• Standard formulation
• A budget-friendly alternative to liquid
• Easy scale-up using larger package sizes

Nota de preparación

1. Suspend 15.5 g in 1 L of distilled water.
2. Autoclave for 15 minutes at 121°C.

Reconstitución

Stir to suspend 15.5g powder in 1L water. Autoclave for 15 minutes at 121C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable