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Key Documents

L6511

Sigma-Aldrich

Lipopolysaccharides from Salmonella enterica serotype typhimurium

purified by phenol extraction

Sinónimos:

LPS

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About This Item

Número MDL:
Código UNSPSC:
12352201
NACRES:
NA.25

origen biológico

Salmonella enterica (Serotype typhimurium)

Nivel de calidad

formulario

lyophilized powder

purificado por

phenol extraction

impurezas

<3% Protein (Lowry)

color

white to faint yellow

solubilidad

water: 4.90-5.10 mg/mL, slightly hazy to turbid, colorless to faintly yellow

Condiciones de envío

ambient

temp. de almacenamiento

2-8°C

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Descripción general

This product is phenol extracted from Salmonella typhimurium. The source strain is ATCC 7823. This LPS has been used to induce NOS in rats and guinea pigs.

Aplicación

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

Acciones bioquímicas o fisiológicas

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Nota de preparación

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Otras notas

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Pictogramas

Exclamation mark

Palabra de señalización

Warning

Frases de peligro

Clasificaciones de peligro

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

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Sanjoy Kumar Bhunia et al.
Carbohydrate research, 345(17), 2542-2549 (2010-10-12)
A water-soluble polysaccharide, isolated from the hot water extract of the fruiting bodies of an edible mushroom, Lentinus squarrosulus (Mont.) Singer was found to contain d-galactose, l-fucose, and d-glucose in a molar ratio of nearly 1:1:5. Structural investigation of the
C Marcq et al.
Poultry science, 90(1), 59-67 (2010-12-24)
In this study, Salmonella enterica serovar Typhimurium challenge models were tested to identify the best conditions under which to perform the experimental infection of 3-wk-old broilers. Such a model would be useful to study the efficiency of therapeutic treatments that
Alejandro Carrillo-Jimenez et al.
Frontiers in cellular neuroscience, 12, 313-313 (2018-10-10)
Microglia, the resident immune cells of the brain, have multiple functions in physiological and pathological conditions, including Alzheimer's disease (AD). The use of primary microglial cell cultures has proved to be a valuable tool to study microglial biology under various
Li Liang et al.
Scientific reports, 3, 1043-1043 (2013-01-11)
Current serological diagnostic assays for typhoid fever are based on detecting antibodies against Salmonella LPS or flagellum, resulting in a high false-positive rate. Here we used a protein microarray containing 2,724 Salmonella enterica serovar Typhi antigens (>63% of proteome) and
Andrej Kovac et al.
Journal of neuroinflammation, 8, 139-139 (2011-10-15)
Brain microvascular pericytes are important constituents of the neurovascular unit. These cells are physically the closest cells to the microvascular endothelial cells in brain capillaries. They significantly contribute to the induction and maintenance of the barrier functions of the blood-brain

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