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Merck

L2380

Sigma-Aldrich

Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia)

lyophilized powder

Sinónimos:

Bandeirea simplicifolia agglutinin, BS-I

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About This Item

Código UNSPSC:
12352202
NACRES:
NA.32

formulario

lyophilized powder

Nivel de calidad

potencia

<20 μg per mL agglutination activity (using human blood group B erythrocytes)

composición

Protein, ≥90% biuret

impurezas

salt, free

temp. de almacenamiento

2-8°C

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Acciones bioquímicas o fisiológicas

BS-I has a major affinity for terminal α-D-galactosyl residues with a secondary affinity for terminal N-acetyl-α-D-galactosaminyl residues.

Otras notas

BS-I is a tetrameric lectin consisting of two types of subunits designated A and B. There are five BS-I isolectins with different subunit composition: BSI-B4, BSI-AB3, BSI-A2B2, BSI-A3B and BSI-A4. BSI-B4 is blood group B specific and has an exclusive affinity for terminal α-D-galactosyl residues, whereas BSI-A4 has blood group A specificity and has a major affinity for terminal N-acetyl-α-D-galactosaminyl residues.

Nota de preparación

Purified by affinity chromatography using the method of Hayes, C. E., and Goldstein, I. J., J. Biol. Chem., 249, 1904 (1974).

Nota de análisis

Agglutination activity is expressed in μg/mL and is determined from serial dilutions of a 1 mg/mL solution using phosphate buffered saline, pH 6.8, containing, for each lectin, calcium, magnesium, and manganese at different concentrations. This activity is the lowest concentration to agglutinate a 2% suspension of appropriate erythrocytes after 1 hr incubation at 25 °C.

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)


Certificados de análisis (COA)

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