L2380
Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia)
lyophilized powder
Sinónimos:
Bandeirea simplicifolia agglutinin, BS-I
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About This Item
Código UNSPSC:
12352202
NACRES:
NA.32
Productos recomendados
Formulario
lyophilized powder
Nivel de calidad
potencia
<20 μg per mL agglutination activity (using human blood group B erythrocytes)
composición
Protein, ≥90% biuret
impurezas
salt, free
temp. de almacenamiento
2-8°C
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Categorías relacionadas
Acciones bioquímicas o fisiológicas
BS-I has a major affinity for terminal α-D-galactosyl residues with a secondary affinity for terminal N-acetyl-α-D-galactosaminyl residues.
Otras notas
BS-I is a tetrameric lectin consisting of two types of subunits designated A and B. There are five BS-I isolectins with different subunit composition: BSI-B4, BSI-AB3, BSI-A2B2, BSI-A3B and BSI-A4. BSI-B4 is blood group B specific and has an exclusive affinity for terminal α-D-galactosyl residues, whereas BSI-A4 has blood group A specificity and has a major affinity for terminal N-acetyl-α-D-galactosaminyl residues.
Nota de preparación
Purified by affinity chromatography using the method of Hayes, C. E., and Goldstein, I. J., J. Biol. Chem., 249, 1904 (1974).
Nota de análisis
Agglutination activity is expressed in μg/mL and is determined from serial dilutions of a 1 mg/mL solution using phosphate buffered saline, pH 6.8, containing, for each lectin, calcium, magnesium, and manganese at different concentrations. This activity is the lowest concentration to agglutinate a 2% suspension of appropriate erythrocytes after 1 hr incubation at 25 °C.
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, type N95 (US)
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