H9773

Higromicina B from Streptomyces hygroscopicus

lyophilized powder, suitable for plant cell culture, BioReagent

• Sinónimos: Hygromycin
• Fórmula empírica (notación de Hill): C₂₀H₃₇N₃O₁₃
• Número de CAS: 31282-04-9
• Peso molecular: 527.52
• Número CE: 250-545-5
• Número MDL: MFCD06795479
• Código UNSPSC: 12352207
• ID de la sustancia en PubChem: 329815270
• NACRES: NA.72

Propiedades

• product name: Higromicina B from Streptomyces hygroscopicus, suitable for plant cell culture, BioReagent, ≥60% (HPLC), lyophilized powder
• origen biológico: Streptomyces hygroscopicus
• Nivel de calidad: 200
• Línea del producto: BioReagent
• formulario: lyophilized powder
• purificado por: ion-exchange chromatography
• concentración: ≥60% (HPLC)
• técnicas: cell culture | plant: suitable
• color: faintly brown to brown; white to beige
• solubilidad: H₂O: soluble 50 mg/mL; ethanol: soluble; methanol: soluble
• espectro de actividad antibiótica: fungi
• aplicaciones: agriculture
• Modo de acción: protein synthesis | interferes
• temp. de almacenamiento: 2-8°C
• cadena SMILES: CN[C@H]1C[C@@H](N)[C@H](O)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O)[C@@H]3O[C@]4(O[C@H]([C@H](N)CO)[C@H](O)[C@H](O)[C@H]4O)O[C@H]23)[C@@H]1O
• InChI: 1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6-,7+,8-,9+,10-,11+,12-,13+,14-,15-,16+,17+,18-,19+,20+/m1/s1
• Clave InChI: GRRNUXAQVGOGFE-XKIAHZFYSA-N

Descripción

Descripción general

Chemical structure: aminoglycoside

Hygromycin B is an antibiotic produced by the bacterium Streptomyces hygroscopicus. It is an aminoglycoside that kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis.

Aplicación

Hygromycin B is an aminoglycoside antibiotic isolated from Streptomyces hygroscopicus. It has been used to study protein synthesis at the level of the 70S ribosome translocation and mRNA template misreading, as an antiviral agent by selectively penetrating cells rendered permeable by virus infection and inhibiting translation, and as a selection agent for hygromycin resistance gene transformed cells. It is recommended for use as a selection agent at 100-800 μg/mL, specifically at 100 μg/mL for prokaryotes, 200 μg/mL for lower eukaryotes and 150-400 μg/mL for higher eukaryotes.

Hygromycin B from Streptomyces hygroscopicus is used for gene cloning and ectopic expression. It is also used in MS medium for the in vitro screening of transgenic plants.

Acciones bioquímicas o fisiológicas

Hygromycin B is used in mutagenesis or for selection of gene expression and complementation constructs in C. jejuni. It is also adopted as a marker in other approaches for manipulating, introducing or deleting DNA in C. elegans. It can be inactivated by Aminoglycoside 4-phosphotransferase-Ia (APH(4)-Ia)/Hygromycin B phosphotransferase (Hph) through phosphorylation. The amino function of this antibiotic may be involved in a general acid-base catalysis performed by the ribozyme, acting as proton acceptors/donors.

Modo de acción: El producto actúa inhibiendo la síntesis de proteínas al inducir la lectura errónea de la plantilla de ARNm en procariotas, con la capacidad de inhibir la traducción.

Espectro antimicrobiano: La higromicina B actúa contra bacterias, hongos y células eucariotas superiores.

Precaución

Hygromycin B products should be stored as supplied at 2-8°C, and the dry solid is table for at least 5 years if stored at 2-8°C. It is stable at 37°C for 30 days. (For solutions) This solution is stable as supplied for two years if stored at 2-8°C.

Nota de preparación

This product is purified by ion exchange chromatography. Hygromycin B is soluble in water at concentrations >50 mg/mL, and also soluble in methanol or ethanol. Solutions should be sterilized by filtration, not by autoclaving. Additionally, Hygromycin B solutions have been reported to lose activity on freezing, and since they are stable refrigerated, freezing should be avoided.

Información de seguridad

• Pictogramas: GHS06
• Palabra de señalización: Danger
• Frases de peligro: H300 + H310 + H330
• Consejos de prudencia: P260 - P262 - P264 - P280 - P302 + P352 + P310 - P304 + P340 + P310
• Clasificaciones de peligro: Acute Tox. 1 Inhalation - Acute Tox. 2 Dermal - Acute Tox. 2 Oral
• Código de clase de almacenamiento: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable