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Merck

H8889

Sigma-Aldrich

Histopaque®-1077 Hybri-Max

liquid, sterile-filtered, BioReagent, suitable for hybridoma

Sinónimos:

Histopaque 1077

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About This Item

Código UNSPSC:
12352201
NACRES:
NA.75

esterilidad

sterile-filtered

Nivel de calidad

Línea del producto

BioReagent

formulario

liquid

técnicas

cell culture | hybridoma: suitable
single cell analysis: suitable

impurezas

endotoxin, tested

densidad

1.077 at 25 °C (relative density)(lit.)

temp. de almacenamiento

2-8°C

Descripción general

Histopaque®-1077 is a solution comprising polysucrose and sodium diatrizoate, adjusted to a density of 1.077 +/- 0.001 g/cm3. This medium facilitates rapid recovery of viable lymphocytes from small volumes of whole blood. This technique is suitable for use in cell-mediated lympholysis and human lymphocyte antigen (HLA)2 typing. It may also be employed as the initial isolation step before the enumeration of T-, B-, and null lymphocytes. It is used to create a density medium for the purification of lymphocytes and other mononuclear cells.

Aplicación

Histopaque®-1077 Hybri-Max has been used to isolate:
  • peripheral blood mononuclear cells (PBMCs) from heparinized blood by gradient centrifugation
  • immune cells (lymphocytes) from peripheral blood
  • PBMCs from blood

Información legal

Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany
Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

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Pictogramas

Health hazard

Palabra de señalización

Danger

Frases de peligro

Clasificaciones de peligro

Resp. Sens. 1 - Skin Sens. 1

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


Certificados de análisis (COA)

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