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Merck
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Key Documents

F5415

Sigma-Aldrich

Ficoll® solution

Type 400, 20% in H2O

Sinónimos:

Ready-to-use density gradient media

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About This Item

Número de CAS:
26873-85-8
Número MDL:
MFCD00081599
Código UNSPSC:
12352207
NACRES:
NA.75

esterilidad

0.2 μm filtered

Nivel de calidad

200

tpo

Type 400

formulario

liquid

concentración

20% in H2O

color

clear colorless

solubilidad

water: soluble

densidad

1.055-1.075 g/mL

aplicaciones

hematology
histology

temp. de almacenamiento

2-8°C

Categorías relacionadas

Descripción general

Ficoll® Solution is a highly branched polymer of sucrose, also known as polydisperse mixtures of dextran. Ficoll® molecules are inert and not re-absorbed by proximal tubules. The structure is open and deformable, and not a rigid sphere thereby becoming a preferred choice in “crowding” experiments.
Used in electrophoresis and as a hapten carrier. Most commonly used to prepare density gradients.

Aplicación

Ficoll® has been used in


  • Isolation of cord blood mononuclear cells
  • Isolation of CD133 negative cells
  • Isolation and expansion of circulating endothelial progenitor cells.

Otras notas

A nonionic synthetic polymer of sucrose.

Información legal

Ficoll is a registered trademark of Cytiva

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
SLCL2553
SLCJ9378
SLCH6817
SLCH3511
SLCH0230

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Xiaoliang Wu et al.
Shock (Augusta, Ga.), 51(6), 718-724 (2018-08-07)
The aim of this study was to investigate the correlation between plasma sphingosine-1-phosphate (S1P) and ceramide concentrations in sepsis, and the possible mechanisms for altered expression. Plasma S1P and ceramide concentrations were measured by HPLC-ESI-MS/MS. HLA-DR (human leukocyte antigen-DR) expression
Circulation enrichment of functional endothelial progenitor cells by infantile phototherapy.
Siavashi V, et al.
Journal of Cellular Biochemistry, 118(2), 330-340 (2017)
Juliane Schmidt et al.
PloS one, 6(3), e18184-e18184 (2011-03-30)
The characterization of factors contributing to the formation and development of surface-associated bacterial communities known as biofilms has become an area of intense interest since biofilms have a major impact on human health, the environment and industry. Various studies have
Effect of mononuclear cells on morphometric of kidney parameters in hyperglycemic rat.
Salmani F, et al.
Annals of Experimental Biology, 2(3), 1-7 (2014)
Analysis of Structural and Functional Glomerular Changes in Hyperglycemic Rats that Treated with Human Umbilical Cord Blood Derived CD133 Negative Ccells.
Feizi F, et al.
Physical Chemistry, 1(1) (2014)
Ver todos los artículos relacionados

Protocolos

Hematopoietic Stem Cell Culture Protocols

Step-by-step hematopoietic stem cell culture protocols for isolation, expansion and differentiation of CD34+ hematopoietic progenitor cells including CFU assays. It can be cultured under defined conditions designed either to promote self-renewal and increase the number of primitive cells.

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