Saltar al contenido
Merck
Todas las fotos(1)

Key Documents

F2637

Sigma-Aldrich

Polisacarosa 400

BioXtra, for molecular biology, lyophilized powder

Sinónimos:

Polysucrose 400, Ficoll 400

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Todas las fotos(1)

About This Item

Número de CAS:
26873-85-8
Número MDL:
MFCD00081599
Código UNSPSC:
12352200
NACRES:
NA.52

origen biológico

non-animal source

Nivel de calidad

200

grado

Molecular Biology
for molecular biology

Línea del producto

BioXtra

formulario

lyophilized powder

actividad extraña

DNase (Exonuclease), NICKase (Endonuclease), RNase and Protease, none detected

temp. de almacenamiento

room temp

¿Está buscando productos similares? Visita Guía de comparación de productos

Descripción general

Ficoll® 400 is a non-ionic synthetic polymer of sucrose used for cell separation and organ isolation.

Aplicación

Suitable for
  • hybridization experiments to reduce non-specific binding of labeled probes to the membrane
  • preparation of DNA loading buffer (15% Ficoll®, 0.25% bromophenol blue and 0.25% xylene cyanol FF
  • separation of cells with similar density but different size (PBMC from human peripheral blood, bone marrow and umbilical cord blood)
  • producing density gradients to separate subcellular components
  • vitrification of cell and tissue samples.
  • to compare the effect of different perfusates on Hypoxic pulmonary vasoconstriction (HPV).
Usado para separación de célula y aislamiento de orgánulos.

Características y beneficios

  • free from DNases, RNases, phosphatases and proteases
  • preserves functional and morphological integrity of the separated cells

Otras notas

Un polímero sintético y no iónico de la sucrosa.

Información legal

Ficoll is a registered trademark of Cytiva

Producto relacionado

Referencia del producto
Descripción
Precios

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Gaye Cetinkaya et al.
Cryobiology, 63(3), 292-297 (2011-10-25)
Preservation of cell and tissue samples from endangered species is a part of biodiversity conservation strategy. Therefore, setting up proper cell and tissue cryopreservation methods is very important as these tissue samples and cells could be used to reintroduce the
Hae Young Yoo et al.
Pulmonary circulation, 3(2), 396-405 (2013-09-10)
Hypoxic pulmonary vasoconstriction (HPV) is a compensatory physiological mechanism in the lung that optimizes the matching of ventilation to perfusion and thereby maximizes gas exchange. Historically, HPV has been primarily studied in isolated perfused/ventilated lungs; however, the results of these
Ivana Gessara et al.
Stem cell reports, 16(4), 784-796 (2021-03-20)
The ability to genetically manipulate organisms has led to significant insights into functional genomics in many species. In birds, manipulation of the genome is hindered by the inaccessibility of the one-cell embryo. During embryonic development, avian primordial germ cells (PGCs)
Weihua Fei et al.
PLoS genetics, 7(7), e1002201-e1002201 (2011-08-11)
Lipid droplets (LDs) are important cellular organelles that govern the storage and turnover of lipids. Little is known about how the size of LDs is controlled, although LDs of diverse sizes have been observed in different tissues and under different
Oindrila Bhattacharya et al.
Plant methods, 16, 131-131 (2020-09-29)
Chloroplasts are critical organelles that perceive and convey metabolic and stress signals to different cellular components, while remaining the seat of photosynthesis and a metabolic factory. The proteomes of intact leaves, chloroplasts, and suborganellar fractions of plastids have been evaluated
Ver todos los artículos relacionados

Protocolos

Northern and Southern Blot Protocols & Introduction

An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico