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H8162

Millipore

HIS-Select® Cobalt Affinity Gel

(1:1 suspension in a 30% ethanol solution)

Sinónimos:

cobalt affinity gel, cobalt charged agarose

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About This Item

Código UNSPSC:
12352200
NACRES:
NA.56

Nivel de calidad

200

formulario

(1:1 suspension in a 30% ethanol solution)

caducidad

1 yr (when stored properly.)

técnicas

protein purification: suitable

matriz

6% Beaded Agarose

capacidad

>15 mg/mL, agarose binding capacity (protein)(with an approx. 30 kDa protein)

temp. de almacenamiento

2-8°C

Categorías relacionadas

Descripción general

The HIS-Select Cobalt Affinity Gel is a quadridentate chelate on beaded agarose that is charged with cobalt. The gel is designed to specifically bind histidine-containing proteins / His-tag proteins. It is selective for recombinant proteins with histidine tags and exhibits low non-specific binding of other proteins.
HIS-Select Cobalt Affinity Gel is an immobilized metal-ion affinity chromatography (IMAC) product. The matrix for this affinity gel is 6% beaded agarose. The selectivity can be modulated with the inclusion of imidazole during chromatography. HIS-Select Cobalt Affinity Gel is durable and can capture recombinant proteins with histidine tags at a high flow rate. Recombinant proteins with His tags are bound using native, denaturing, or mild reducing conditions. The capacity of this affinity gel is typically >15 mg/mL of packed gel, determined with a His-tagged protein of molecular mass ~30 kDa.

Aplicación

HIS-Select Cobalt Affinity Gel allows for high purity, low non-specific binding, and high binding capacity of protein. HIS-Select Cobalt Affinity Gel also works well for purification of histidine-tagged proteins / His-tag proteins in native, denaturing, or mild reducing conditions. HIS-Select™ Cobalt Affinity Gel has been used to purify His-tagged proteins.

Forma física

1:1 suspension in a 30% ethanol solution

Información legal

HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany
HiScreen is a trademark of Cytiva

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Pull-down assays, reagents, and protocols for investigating in vitro protein-protein interactions using affinity or GST pull-down, tandem affinity purification (TAP), and co-immunoprecipitation methods.

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